We narrowed to 31,565 results for: REP
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Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minProAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only
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pPEPY-PF6-lacI
Plasmid#85589PurposeWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniaeDepositorInsertlacI
ExpressionBacterialPromoterPF6Available SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minTK
Plasmid#211516PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterSV40 and minTKAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas3
Plasmid#209427PurposePlasmid for CASCADE-Cas3 based genome engineering of streptomycetesDepositorInsertcodon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
UseCRISPR and Synthetic BiologyPromoterPtipA; crRNA under control of PermE*Available SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hMYC
Plasmid#20723DepositorAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dTomato-ORP9-PH
Plasmid#214268PurposeMammalian expression of red fluorescent PI4P reporter dTomato-ORP9-PHDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-OgNLuc
Plasmid#70186PurposeConstiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionPromoteralpha-crystallin and cmlc2Available SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOCT4
Plasmid#20726DepositorAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
7Gli:GFP
Plasmid#110494Purposereporter of Hedgehog signaling activityDepositorInsert7 repeats of the Gli binding site GAACACCCA
UseLentiviralExpressionMammalianPromoter7 repeats of Gli biding siteAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
Plasmid#113864PurposeSingle pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.DepositorInsertsPTCH1 (hedgehog dependent promoter)
mTurquoise2
UseMule gateway entry vectorExpressionMammalianAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq)
Plasmid#124432PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)DepositorInsertFlipGFP(Casp3 cleavage seq)
UseZebrafish (good expression)Available SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG_hPPP1R15B_4-713_mCherry_UK1298
Plasmid#80707Purposemammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)DepositorInserthuman PPP1R15B (PPP1R15B Human)
TagsmCherryExpressionMammalianMutationCDS 4-713 no stop codonPromoterCMVAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
TagsStrep(II)-tagExpressionBacterialPromoterT7 PromotorAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-1xStop
Plasmid#164411PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianPromoterEF1-alphaAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCRISPomyces-2BD
Plasmid#234429PurposePlasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified dCas9(D10A, H840A) with polyaspartate linking
ExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
MutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEC819
Plasmid#190815PurposeCRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9-XTEN-rpoA
UseCRISPR and Synthetic BiologyTagsXTEN (linker), followed by the N-terminal domain …PromoterSP30Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX458_3XHA_dFnCas9_KRAB_T2A_eGFP
Plasmid#200389Purpose3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsertPX458_3XHA_dFnCas9_KRAB_eGFP
UseCRISPRTags3xHA-NLS and SV40NLS-KRAB-T2A-EGFPExpressionMammalianPromoterCBHAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AcGFP1-ORP9-PH
Plasmid#214266PurposeMammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PHDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only