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pYTK066

Plasmid

#65173

Purpose

Encodes tTDH1 as a Type 4b part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

tTDH1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK014

Plasmid

#65121

Purpose

Encodes pTEF2 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pTEF2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK023

Plasmid

#65130

Purpose

Encodes pRNR2 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pRNR2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK025

Plasmid

#65132

Purpose

Encodes pRAD27 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pRAD27

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK026

Plasmid

#65133

Purpose

Encodes pPSP2 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pPSP2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK030

Plasmid

#65137

Purpose

Encodes pGAL1 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pGAL1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK011

Plasmid

#65118

Purpose

Encodes pPGK1 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pPGK1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRPR1_c4gRNA_RPR1t

Plasmid

#64382

Purpose

encodes c4 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c4 gRNA

Expression

Yeast

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c8gRNA_RPR1t

Plasmid

#64386

Purpose

encodes c8 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c8 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pAGM1263

Plasmid

#47985

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pICH41258

Plasmid

#47987

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAGM1299

Plasmid

#47988

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pICH41246

Plasmid

#47992

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-TD!TA

Plasmid

#48655

Purpose

Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGA-01-47

Plasmid

#252303

Purpose

MagLOV2 for constitutive expression in E. Coli

Depositor

Harrison Steel

Article

Abrahams et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

MagLOV 2

Tags

6xHis-tag

Expression

Bacterial

Promoter

J23119

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pRedCas9

Plasmid

#240153

Purpose

Expresses lambda red machinery and cas9

Depositor

Morten Norholm

Article

Gude et al bioRxiv 2025.06.19.660532

Inserts

gam
beta
exo
cas9

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pJL31

Plasmid

#227428

Purpose

Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

Depositor

Pierre Santucci

Article

Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

Insert

mWasabi

Use

CRISPR

Expression

Bacterial

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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