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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0782

Plasmid

#133514

Purpose

Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX

Expression

Yeast

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC-20XUAS-IVS-PhiC31

Plasmid

#133565

Purpose

Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Publication

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Promoter

20X UAS

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pREVTRE-AID

Plasmid

#121067

Purpose

Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio

Depositor

Randy Poon

Publication

Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

Type

Empty backbone

Use

Retroviral

Tags

AID

Expression

Mammalian

Promoter

TRE

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDM1513

Plasmid

#108998

Purpose

safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, n…

Tags

GFP

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDM1515

Plasmid

#109000

Purpose

safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, c…

Tags

GFP

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDM1514

Plasmid

#108999

Purpose

safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, n…

Tags

mCherry

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMpGWB212

Plasmid

#68603

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSB4A5 JQ929582

Plasmid

#38169

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pMMBrelAGent

Plasmid

#45474

Depositor

Michele Swanson

Publication

Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

Insert

relA

Expression

Bacterial

Mutation

truncated at EcoRI/PstI sites of RelA, but metabo…

Promoter

ptac

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pT-TK

Plasmid

#31549

Depositor

Yegor Vassetzky

Publication

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LoxP

Use

Luciferase

Expression

Mammalian

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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CBS-7171

Plasmid

#253635

Purpose

Ba1Cas12a3 expression

Depositor

Chase Beisel

Publication

Dmytrenko et al Nature. 2026 Jan;649(8099):1312-1321. do

Insert

Ba1Cas12a3 E. coli codon optimized C-terminal His tag

Tags

His

Expression

Bacterial

Mutation

N/A

Promoter

T7

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDL1403

Plasmid

#226635

Purpose

This plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.

Depositor

Andrew Camilli

Publication

Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

tfox
qstR

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G

Plasmid

#220894

Purpose

Express haA3A-CBE-G (with SpCas9) in mammalian cells

Depositor

Dali Li

Publication

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

haA3A-CBE-G

Expression

Mammalian

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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DinoIII

Plasmid

#128100

Purpose

Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.

Depositor

Senjie Lin

Publication

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

RNA complex & upstream/downstream of LHC

Expression

Bacterial

Promoter

Inserts are potential promoters

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLB038 - PHS1∆P

Plasmid

#136285

Purpose

entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

Depositor

Alexis Maizel

Publication

Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

Insert

PHS1∆P

Expression

Bacterial

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0785

Plasmid

#133507

Purpose

Expression of mCherry tagged PCNA to monitor DNA replication

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0620

Plasmid

#133506

Purpose

Expression of eGFP tagged PCNA to monitor DNA replication

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Publication

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

Plasmid

#108849

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

nMag

Expression

Mammalian

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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