We narrowed to 823 results for: Anti-CRISPR

1. pLL5 sgRNA(MS2)-MS2-hA3a D131A

   Plasmid

   #112131

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a D131A

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a D131A mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D131A mutant in hA3a

   Promoter

   hU6,CBh

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHybr_r2a>m2a(silent)-srt-his

   Plasmid

   #124807

   Purpose

   HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG2a (Fc silent)
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Mutation

   L234A/L235A/N297A

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHybr_r2a>Fab-srt-his

   Plasmid

   #124810

   Purpose

   HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   rat IgG2a Hinge-G4S-Sortag-Histag
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial

   Mutation

   Promoter

   pUC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHisIHFαIHFβ

   Plasmid

   #149384

   Purpose

   Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Inserts

   Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
   Pseudomonas aeruginosa PA14 IHFbeta
   

   Use

   Tags

   6xHis tag and HRV3C cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pStrepIHFb

   Plasmid

   #149385

   Purpose

   Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
   

   Use

   Tags

   HRV3C cleavage site and StrepII-tag with

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PROM5_MpU6

   Plasmid

   #136120

   Purpose

   Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM5_MpU6
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-TetON-dual-SunTag-Hygro

   Plasmid

   #121119

   Purpose

   Endogenous gene activation with the SunTag CRISPR activation system

   Depositor

   Pablo Navarro

   Article

   Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

   Insert

   dCas9-GCN4x10_scFv-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAS088

   Plasmid

   #211502

   Purpose

   AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Type

   Empty backbone

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAS006

   Plasmid

   #211506

   Purpose

   AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Type

   Empty backbone

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115214

    Purpose

    Expresses PauCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PauCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge