We narrowed to 9,223 results for: Coli

1. pRAR114

   Plasmid

   #185050

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region E8A mutation, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E8A amino acid mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRAR089-L36p

   Plasmid

   #185042

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L36p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRAR090-L36

   Plasmid

   #185043

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L36
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRAR091-L31p

   Plasmid

   #185044

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31p
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218767

   Purpose

   encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-pAAFRS-9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y37G, L71V, D182C, F183Y, L186C, D265R

   Promoter

   tacI

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCSG7-10xHis-ecRNHI*

   Plasmid

   #219523

   Purpose

   bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

   Depositor

   Arthur Palmer

   Article

   Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

   Insert

   cysteine-free variant of the E. coli ribonuclease HI gene
   

   Use

   Tags

   N terminal 10xHis Tag

   Expression

   Bacterial

   Mutation

   Cysteine free variant

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBN095

   Plasmid

   #173765

   Purpose

   express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   delete motif 3

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBN215

   Plasmid

   #167962

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBN216

   Plasmid

   #167963

   Purpose

   express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E

   Depositor

   James Naismith

   Article

   Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

   Insert

   E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   Y713E, Y715E, Y717E, Y718E

   Promoter

   araBAD

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBN217

    Plasmid

    #167964

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y708E, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBN261

    Plasmid

    #167966

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y713E, Y715E, Y717E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBN262

    Plasmid

    #167967

    Purpose

    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Y708E, Y713E, Y715E, Y717E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBRC901

    Plasmid

    #167948

    Purpose

    express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBN008

    Plasmid

    #167949

    Purpose

    express E. coli E69 (O9a:K30) Wzc with K540M mutation

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBN202

    Plasmid

    #167950

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, Y713E, Y715E, Y717E, Y718E

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBN093

    Plasmid

    #167951

    Purpose

    express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation

    Depositor

    James Naismith

    Article

    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

    Insert

    E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    K540M, delete motif 3

    Promoter

    araBAD

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AlkB-D135G

    Plasmid

    #160434

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Glycine; deleted the…

    Promoter

    T7

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVR

    Plasmid

    #84718

    Purpose

    E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilis

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    5' UTR of queC gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCML 314

    Plasmid

    #98584

    Purpose

    pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Inserts

    CsrA
    csrB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacO

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMART-Ala1

    Plasmid

    #65814

    Purpose

    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    NADPH Alanine Dehydrogenase (ald Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D196A, L197R

    Promoter

    Insulated waaHp from E. coli

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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