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We narrowed to 9,400 results for: Coli

Showing: 921 - 940 of 9400 results

  1. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  3. pJMP3667

    Plasmid
    #222353
    Purpose
    Expression vector with GFP expressed under PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli.
    Depositor
    Jason Peters
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PabstBR
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222353-plasmid-map-sequence-id-439080

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  4. pCASCADE-gltA1-gltA2-udhA-GapAP1

    Plasmid
    #87151
    Purpose
    gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-GapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87151-plasmid-map-sequence-id-174940

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  5. pGLU_116

    Plasmid
    #225796
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225796-plasmid-map-sequence-id-447051

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  6. pGLU_80

    Plasmid
    #225781
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225781-plasmid-map-sequence-id-447019

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  7. pGLU_98

    Plasmid
    #225811
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225811-plasmid-map-sequence-id-446973

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  8. pKP864

    Plasmid
    #167658
    Purpose
    Express colicinB-His6
    Depositor
    Kathleen Postle
    Insert
    cba-his6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6
    Promoter
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167658-plasmid-map-sequence-id-337304

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  9. pcpBpaRS_v2

    Plasmid
    #196485
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196485-plasmid-map-sequence-id-388866

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  10. pET19b-EcMscL-TEV-mEGFP

    Plasmid
    #165097
    Purpose
    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.
    Depositor
    Neha Kamat
    Article
    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p
    Insert
    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    Use
    Synthetic Biology
    Tags
    monomeric Enhanced Green Fluorescent Protein
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    165097-plasmid-map-sequence-id-324525

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  11. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Promoter
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

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  12. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

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  13. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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  14. pLPE1

    Plasmid
    #209981
    Purpose
    Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (pMB1_ampR_B0017)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 13, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209981-plasmid-map-sequence-id-419436

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  15. pLPE2

    Plasmid
    #209982
    Purpose
    Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (ampR_B0017)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 13, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209982-plasmid-map-sequence-id-419966

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  16. pLPE3

    Plasmid
    #209985
    Purpose
    Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    E. coli shuttle part (pMB1_cat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 13, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209985-plasmid-map-sequence-id-419384

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  17. pGBW-m4136458

    Plasmid
    #149531
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149531-plasmid-map-sequence-id-297078

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  18. pdUA-lacIN

    Plasmid
    #141079
    Purpose
    Plasmid vector for testing DNA binding proteins by the I-Block assay.
    Depositor
    Antal Kiss
    Article
    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28
    Type
    Empty backbone
    Use
    Vector for the i-block assay designed to study dn…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Escherichia coli lacIN promoter
    Available Since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    141079-plasmid-map-sequence-id-310860

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  19. pICSL80036

    Plasmid
    #68259
    Purpose
    Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)
    Depositor
    Nicola Patron
    Article
    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:
    Insert
    Coding sequence, hygromycin phophotransferase II (Escherichia coli)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68259-plasmid-map-sequence-id-119204

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Showing: 921 - 940 of 9400 results