We narrowed to 2,493 results for: ERK

1. pLAMP1-miRFP718nano

   Plasmid

   #195749

   Purpose

   LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   LAMP1-miRFP718nano
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pActin-miRFP718nano

   Plasmid

   #195745

   Purpose

   Human beta-actin labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   miRFP718nano-human beta-actin
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIR-Fb6m

   Plasmid

   #184680

   Purpose

   NIR-Fb to spike-Cov-19 RBD

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fb6m (S Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. cmamxA1-GFP

   Plasmid

   #107195

   Purpose

   Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   cmannexin A1
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD/HisB-PAiRFP2

   Plasmid

   #44271

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

   Insert

   PAiRFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

   Promoter

   pBAD

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAL4-sfGFP(158-238)

   Plasmid

   #220744

   Purpose

   GAL4-sfGFP(158-238) construct expression in mammlian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

   Insert

   GAL4-sfGFP(158-238)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNLS-VP16-NIR-Fb(718)

   Plasmid

   #220745

   Purpose

   NLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

   Insert

   NIR-Fb(718)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   NLS-VP16

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGAL4-NIR-FbLAG30

   Plasmid

   #220746

   Purpose

   GAL4-NIR-FbLAG30 construct expression in mammlian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

   Insert

   GAL4-NIR-FbLAG30
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-NIR-FbLAG30

   Plasmid

   #220748

   Purpose

   NIR-FbLAG30 expression in bacteria.

   Depositor

   Vladislav Verkhusha

   Article

   Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

   Insert

   NIR-FbLAG30
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Bacterial

   Mutation

   For bacterial cytoplasmic expression, the pelB si…

   Promoter

   T7

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-NIR-FbLAG16

    Plasmid

    #220749

    Purpose

    NIR-FbLAG16 expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG16
    

    Use

    AAV and Affinity Reagent/ Antibody

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-NIR-FbLAG30

    Plasmid

    #220750

    Purpose

    NIR-FbLAG30 expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG30
    

    Use

    AAV and Affinity Reagent/ Antibody

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMVd1-NLS-GAL4-iLight-VP16

    Plasmid

    #219549

    Purpose

    NLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight-VP16
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd1

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. eDrTrkB

    Plasmid

    #183997

    Purpose

    Optically controlled eDrTrkB expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkB (NTRK2 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pClathrin-miRFP718nano

    Plasmid

    #195747

    Purpose

    Clathrin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-clathrin light chain
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTubulin-miRFP718nano

    Plasmid

    #195746

    Purpose

    Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human alpha-tubulin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRD415

    Plasmid

    #178197

    Purpose

    Rok _Linker overexpression

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    rok _linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deletion of residues 75-96

    Promoter

    T7

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRD461

    Plasmid

    #178198

    Purpose

    Rok with a C-terminal His-tag overexpression

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    rok 6xHis
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-NLS-QPAS1-VP16

    Plasmid

    #186187

    Purpose

    AAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activation

    Depositor

    Vladislav Verkhusha

    Article

    Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

    Insert

    NLS-QPAS1-VP16
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNIR-Fb21

    Plasmid

    #184679

    Purpose

    NIR-Fb to spike-Cov-19 RBD

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb21 (S Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6xHis(TEV)-Drp1(K569A,K571A)-StrepII

    Plasmid

    #174426

    Purpose

    Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K569A, K571A

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge