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  2. lentiCRISPR - AAVS1 sgRNA

    Plasmid
    #70661
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against AAVS1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pCoofy50

    Plasmid
    #55189
    Purpose
    Mammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    8x His, FLAG, HA, PreScission site (3C protease s…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55189-plasmid-map-sequence-id-85571

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Showing: 921 - 940 of 2333 results