We narrowed to 13,730 results for: TIM;

1. pETM6-G6-mCherry

   Plasmid

   #66533

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter G6

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - G6

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. IRE-nonOpt-TREAT

   Plasmid

   #196922

   Purpose

   The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   non-optimal Renilla luciferase
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSopBC460S

   Plasmid

   #112514

   Purpose

   PsopB driven sopB (catalytically inactive) and sigE

   Depositor

   Olivia Steele-Mortimer

   Article

   Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

   Insert

   sopB (catalytically inactive)/sigE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SopB C460S

   Promoter

   PsopB

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-H10-mCherry

   Plasmid

   #66531

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter H10

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - H10

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-H9-mCherry

   Plasmid

   #66532

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter H9

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - H9

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiReport PU.1 3' UTR

   Plasmid

   #26530

   Depositor

   David Baltimore

   Article

   O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

   Insert

   PU.1 3' UTR (Spi1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pGC4785

   Plasmid

   #113409

   Purpose

   Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

   Depositor

   Guillaume Cambray, Adam Arkin

   Article

   Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

   Insert

   359_31121113_2
   

   Use

   Synthetic Biology

   Tags

   fused to codon deptimized sfGFP with Flexible lin…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-oChD-citrine

   Plasmid

   #50976

   Purpose

   AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   oChD-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   ChD gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUB_SMFLuc0---MS2

   Plasmid

   #210568

   Purpose

   Expresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpins

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-nonoptimal FLuc
   

   Use

   Lentiviral and Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUB_SMFLuc100---MS2

    Plasmid

    #210570

    Purpose

    Expresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpins

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-optimal FLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    UBC human

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFPO-His-G-Ca-FLITS

    Plasmid

    #191455

    Purpose

    Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    His

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2385-Tier1-PhCMV-fastFT

    Plasmid

    #169518

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven fluorescent timer, fast maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS2384-Tier1-PhCMV-medFT

    Plasmid

    #169517

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven fluorescent timer, medium maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)

    Plasmid

    #173462

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)

    Plasmid

    #173460

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS41K-mCherry-mNeonGreen (pJJF003)

    Plasmid

    #173459

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)

    Plasmid

    #173458

    Purpose

    C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. IgK-IFN-luc

    Plasmid

    #14886

    Purpose

    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

    Depositor

    David Baltimore

    Article

    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

    Insert

    IgK-IFN (IFNB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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