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Plasmid#182326PurposeBeta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertbeta2AR-ÎĽDronpa-TagRFP-T (ADRB2 Human)
UseTagsFull-length human beta2 adrenergic receptorExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIIα-μDpTT
Plasmid#182327PurposePKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertRIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
UseTagsFull-length human PKA type 2alpha regulatory subu…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ai-LV (CS-TRE-mOKS-PRE-Ubc-rtTA-I2G)
Plasmid#198058PurposeGeneration of rodent iPS cells from somatic cellsDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-ExRaiAKAR
Plasmid#160724PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.DepositorInsertS3-ExRaiAKAR
UseAAVTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S4-ExRaiCKAR
Plasmid#160725PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.DepositorInsertS4-ExRaiCKAR
UseAAVTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s
Plasmid#107704Purposein vivo voltage imagingDepositorInsertpaQuasAr3-P2A-CheRiff
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-RAB_EKARev
Plasmid#160726PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.DepositorInsertS2-RAB_EKARev
UseAAVTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-RAB_EKARev
Plasmid#160727PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.DepositorInsertS3-RAB_EKARev
UseAAVTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-jRCaMP1a
Plasmid#160728PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-jRCaMP1a
UseAAVTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-Rb1shRNA1
Plasmid#220584PurposeTo inducibly knockdown Rb1 expressionDepositorInsertRb1 shRNA1 (RB1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-Rb1shRNA2
Plasmid#220585PurposeTo inducibly knockdown Rb1 expressionDepositorInsertRb1 shRNA2 (RB1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-Rb1shRNA3
Plasmid#220586PurposeTo inducibly knockdown Rb1 expressionDepositorInsertRb1 shRNA3 (RB1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCA2-DuET
Plasmid#213308PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHCA2 (HCAR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-6xTRE-FLEX-hM4Di.mCherry
Plasmid#169415PurposeActivity-dependent, cre-dependent, temporally restricted inhibitory DREADD expressionDepositorInserthM4Di.mCherry
UseAAVTagsmCherryExpressionMutationhM4Di is Gi-coupled DREADD.Derived from human mus…PromoterMinimal TATAAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
p21-P369S-MEFV
Plasmid#134712PurposeLentiviral expression of human MEFV P369S after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.P369SPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M680I-MEFV
Plasmid#134711PurposeLentiviral expression of human MEFV M680I after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M680IPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M694I-MEFV
Plasmid#134710PurposeLentiviral expression of human MEFV M694I after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M694IPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-S208C/M694V-MEFV
Plasmid#134709PurposeLentiviral expression of human MEFV S208C/M694V after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.S208C/M694VPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p21-M694V-MEFV
Plasmid#134703PurposeLentiviral expression of human MEFV M694V after doxycycline administrationDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMutationp.M694VPromoterTRE2 (Dox-inducible)Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EWSR1::WT1(-KTS)
Plasmid#220565PurposeLentiviral expression of human EWSR1::WT1(-KTS)DepositorUseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only