We narrowed to 5,536 results for: mcs

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pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMV-BC0527-luc2

Plasmid

#214657

Purpose

barcoded assay, Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered NFAT element with barcode 0527

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR:G249A

Plasmid

#79163

Purpose

Expresses inactive WspR G249A under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Mutation

G249A

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MBP-RGG-SmBit-RGG

Plasmid

#176644

Purpose

SmBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-SmBit-RGG

Expression

Bacterial

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Hy_pMT EGFP SV40 pA

Plasmid

#69911

Purpose

Expresses EGFP in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

EGFP

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJEC625

Plasmid

#174368

Purpose

Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMV-BC0482-luc2

Plasmid

#214635

Purpose

barcoded assay, cAMP/Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered CRE element with barcode 0482

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJA317

Plasmid

#74488

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

d1ODC

Use

Lentiviral

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDUET-aaCPRct-A13AMO

Plasmid

#20116

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and Amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pJBL7032

Plasmid

#133873

Purpose

AtzR expression plasmid

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

Insert

AtzR

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7034

Plasmid

#133869

Purpose

AtzA expression plasmid

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

Insert

AtzA

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJA318

Plasmid

#74489

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::d4ODC::wPRE has d4ODC degron

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

d4ODC

Use

Lentiviral

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGH311_wtGFP S65T

Plasmid

#85408

Purpose

lentiviral expression vector for wtGFP S65T

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Pl-B

Plasmid

#79161

Purpose

Expresses the Pl-B biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Pl-B

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRBS wt

Plasmid

#154126

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

wt bacterial rRNAs operon under control of pL promotor

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL7036

Plasmid

#133871

Purpose

AtzC expression plasmid

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

Insert

AtzC

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGH312_wtGFP Q80H

Plasmid

#85409

Purpose

lentiviral expression vector for wtGFP Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP Q80H

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGH314_wtGFP S65T, Q80H

Plasmid

#85407

Purpose

lentiviral expression vector for wtGFP S65T-Q80H

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

wtGFP S65T, Q80H

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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MBP-RGG-LgBit-RGG

Plasmid

#176643

Purpose

LgBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-LgBit-RGG

Expression

Bacterial

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFF19H

Plasmid

#49783

Purpose

expresses hygromycin resistance gene in plant cells

Depositor

Joachim Messing

Article

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Insert

HPH

Use

Plant expression

Promoter

CaMV 35S

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only