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pSG5-p85α-Δbcr

Plasmid

#39500

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 Δbcr (PIK3R1 Bovine)

Use

Eukaryotic

Mutation

Δbcr = deletion of AA134-302

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-p85α_wt

Plasmid

#39501

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 (PIK3R1 Bovine)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSWITCHER-TF1-dsRED

Plasmid

#213043

Purpose

SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

SWITCHER-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

cBH

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER1-TF1-dsRED

Plasmid

#213044

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

cBH

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER2-TF1-dsRED

Plasmid

#213045

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crF1

Plasmid

#213046

Purpose

Guide RNA expression vector with the synthetic crRNA crF1 targeting the SWITCHER construct with the target site TF1

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crF1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crR2

Plasmid

#213047

Purpose

Guide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crR2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLoxP-EGFP-crRNA-entry

Plasmid

#213048

Purpose

SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

LoxP-EGFP-MALAT1-triplex

Use

CRISPR

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM_ΔndhD2.km

Plasmid

#185532

Purpose

a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus

Depositor

Paula Tamagnini

Article

Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

Insert

nptII gene flanked by ndhD2 upstream and downstream regions

Use

Synthetic Biology

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC078

Plasmid

#202212

Purpose

Level1 DNA-Tag with bc066

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc066

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC075

Plasmid

#202210

Purpose

Level1 DNA-Tag with bc063

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc063

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBCC077

Plasmid

#202211

Purpose

Level1 DNA-Tag with bc065

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc065

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-mTurquoise2

Plasmid

#191010

Purpose

Lentiviral expression vector for mTurquoise2

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

mTurquoise2

Use

Lentiviral

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F03R

Plasmid

#198664

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F03L

Plasmid

#198663

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F01R

Plasmid

#198660

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F01L

Plasmid

#198659

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.A02R

Plasmid

#198658

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with FL protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A02L

Plasmid

#198657

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with BF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A01R

Plasmid

#198656

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge