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JPF0520

Plasmid

#124011

Purpose

Encodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCMV-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MSRP5-bio-His

Plasmid

#50805

Purpose

Expresses enzymatically monobiotinylated full length MSRP5

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSRP5

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PMV-ycrtYB

Plasmid

#65343

Purpose

systhesis of crtYB gene in the beta-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtYB

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191183

Purpose

Expression of PPIB sensor in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:PPIB.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMOS031E: Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

Plasmid

#163068

Purpose

Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S669A

Plasmid

#19282

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc S669A (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
mOrange2-Vinculin-23

Plasmid

#57978

Purpose

Localization: Focal Adhesions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vinculin (VCL Human)

Tags

mOrange2

Expression

Mammalian

Mutation

V234L in vinculin

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)

Plasmid

#191142

Purpose

Expression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s17d9b:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYFP-lacY-.008

Plasmid

#140140

Purpose

Evaluating the impact of feedback and low sensor concentration on information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Expression

Bacterial

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pME-nCas9n-P2A-mKate2-pA

Plasmid

#109547

Purpose

Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n and mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PMV-ycrtE

Plasmid

#65341

Purpose

systhesis of crtE gene in the beta-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtE

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MTK6_016

Plasmid

#124058

Purpose

Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

WPRE (extended)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn-FLEX.iGABASnFR.F102Y.Y137L.mRuby3

Plasmid

#112171

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Tags

mRuby3

Mutation

F102Y.Y137L

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJH3577

Plasmid

#179612

Purpose

Pceh-12::Chrimson::Cherry unc-54 3' UTR C.elegans VB-MNs expression of Chrimson::Cherry

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

Chrimson

Tags

Cherry

Expression

Worm

Promoter

Pceh-12

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S502A/669A

Plasmid

#19283

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-myc-FRNK

Plasmid

#74481

Purpose

This plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).

Depositor

J Thomas Parsons

Article

Xiong et al J Cell Biol. 1997 Oct 20;139(2):529-39.

Insert

FRNK (PTK2 Chicken)

Tags

c-myc

Expression

Mammalian

Mutation

FRNK is the C-terminal, noncatalytic domain of th…

Promoter

CMV

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only
RSPH1_pCSdest

Plasmid

#53926

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RSPH1 (RSPH1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
mCerulean3-PTK2-N-10

Plasmid

#55442

Purpose

Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PTK2 (PTK2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
p3E-Bin1b

Plasmid

#109561

Purpose

Multisite gateway vector for 5' tagging with Bin1b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Bin1b (bin1b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mTFP1-FAK-5

Plasmid

#55484

Purpose

Localization: Focal Adhesion Kinase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

FAK

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only