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pcDNA3/hArf5(WT)-HA

Plasmid

#79427

Purpose

Expresses C-terminally HA-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Tags

HA

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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anti-Notch2_B6-pBIOCAM5

Plasmid

#39347

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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PITPbeta-GFP

Plasmid

#58274

Purpose

mammalian expression of GFP-PITP beta

Depositor

Shamshad Cockcroft

Article

Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

Insert

PITP beta (PITPNB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pSL2783 (pTnsC-VP64_Vch)

Plasmid

#200896

Purpose

Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTnsC-VP64

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELF1

Plasmid

#167745

Purpose

Expresses ELF1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELF1 (ELF1 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (T1462A)

Plasmid

#14130

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
p63RhoGEF619-mVenus-N1

Plasmid

#84339

Purpose

Expresses p63 (619 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

p63RhoGEF (619 aa) (ARHGEF25 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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AMPK-β1-FLAG

Plasmid

#40602

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β1 (Prkab1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
MAC_C_MUSK

Plasmid

#187781

Purpose

MAC-tagged gene expression of human MUSK

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

MUSK (MUSK Human)

Expression

Mammalian

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
ECFP-ER-0

Plasmid

#55342

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

ER (CALR Human)

Tags

ECFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
MAC_N_IRF5

Plasmid

#167762

Purpose

Expresses IRF5 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

IRF5 (IRF5 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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R26-H2B-mCherry HR donor vector

Plasmid

#137928

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-mCherry-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pUASTattB-dALFANb(3mut)-mGreenLantern

Plasmid

#219662

Purpose

Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-N2-PKCgamma

Plasmid

#21204

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKC gamma (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

PKC gamma (rat). (Lab plasmid WC 0013)

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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MAC_C_EphA7

Plasmid

#187765

Purpose

MAC-tagged gene expression of human EphA7

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EphA7 (EPHA7 Human)

Expression

Mammalian

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DD-T6B-MUT-YFP

Plasmid

#235144

Purpose

Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused mutant T6B peptide

Use

AAV

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

hSyn

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_C_TYRO3

Plasmid

#187793

Purpose

MAC-tagged gene expression of human TYRO3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

TYRO3 (TYRO3 Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLAG-HA-T6B-MUT-YFP

Plasmid

#235140

Purpose

Expresses the mutant T6B peptide fused with YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mutant T6B peptide

Use

AAV

Tags

FLAG/HA

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

hSyn

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCX-Lyn-SpiCee

Plasmid

#140837

Purpose

mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

Lyn-SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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