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Anti-GST [N100/13R ]

Plasmid

#188214

Purpose

Mammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

Plasmid

#108693

Purpose

For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Inserts

RNASEH2B (RNASEH2B Human)
RNASEH2C (RNASEH2C Human)
RNASEH2A-D34A/D169A

Tags

GST

Expression

Bacterial

Mutation

Shine Dalgarno sequence upstream of start codon, …

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR3DL3.1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156735

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DL3 (KIR3DL3 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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V5-ERG-R367K

Plasmid

#118624

Purpose

Expresses V5 tagged ERG R367K (DNA binding mutant) protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (R367K DNA binding mutant) (ERG Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R367K

Promoter

EF1a

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha1-EGFP

Plasmid

#126463

Purpose

Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

Insert

Myelin oligodendrocyte glycoprotein (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (WT)

Plasmid

#42202

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

c-SRC (SRC Human)

Expression

Mammalian

Mutation

L176F mutation compared to reference sequence NM_…

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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Tet-Inducible Human Caspase-9 Alpha C287A Mutant

Plasmid

#194709

Purpose

Inducible Expression of Human Caspase-9 Alpha C287A Mutant

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

Human Caspase-9 C287A Mutant (CASP9 Human)

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine 287 to Alanine to eliminate prot…

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-Muc1 mAb

Plasmid

#214954

Purpose

Bacterial expression of anti-Muc1 (human) mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-Muc1 mAb heavy chain (MUC1 )
VNp-anti-Muc1 mAb light chain (MUC1 )

Tags

6xHis and VNp

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LILRA1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156866

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LILRA1 (LILRA1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LILRA2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156856

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LILRA2 (LILRA2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-MVP delta607-623aa

Plasmid

#204544

Purpose

Expresses EGFP-tagged MVP delta607-623aa in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Mammalian

Mutation

delta607-623aa

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ULBP1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156597

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ULBP1 (ULBP1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pIG-747pUC19-tNGFR-P2A-CD7

Plasmid

#186086

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD7 (CD7 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA4C-Fc(DAPA)-AviTag-6xHis

Plasmid

#156935

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA4C (SEMA4C Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUltra-Hot-PC

Plasmid

#184466

Purpose

Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53

Plasmid

#196267

Purpose

Mammalian expression of 3xHA tagged wild-type p53

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 (Trp53 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PTPRF-Fc(DAPA)-AviTag-6xHis

Plasmid

#157036

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PTPRF (PTPRF Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LILRA3-Fc(DAPA)-AviTag-6xHis

Plasmid

#156846

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LILRA3 (LILRA3 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBFC0619

Plasmid

#186690

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF2-E79Q-V5-miniTurbo

Plasmid

#207814

Purpose

C-terminal tagged NRF2-E79Q for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF2-E79Q-V5-miniTurbo (NFE2L2 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Mutation

E79Q

Promoter

UBC

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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