162,660 results

1. pENTR-MBD1

   Plasmid

   #47057

   Purpose

   Expresses methyl-CpG binding domain protein 1

   Depositor

   Huda Zoghbi

   Article

   Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

   Insert

   Methyl-CpG Binding Domain Protein 1 (MBD1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pMGE-T7

   Plasmid

   #153021

   Purpose

   Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

   Depositor

   Vito Valiante

   Article

   Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1-Nucleocapsid

   Plasmid

   #179696

   Purpose

   Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

   Depositor

   Mark Arbing

   Insert

   SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His6-TEV protease site

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1a-nSpCas9

   Plasmid

   #171691

   Purpose

   Expresses nSpCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF1a

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIX403_Capture1_APOBEC_HA_P2A_mRuby

   Plasmid

   #183901

   Purpose

   Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   Capture1_APOBEC_HA_P2A_mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

   Plasmid

   #208361

   Purpose

   Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

   Depositor

   David Bennett

   Article

   Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

   Insert

   mCherry-TANDEM-PSAM4-GlyR
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 MKK7b1(K149A)

   Plasmid

   #14674

   Depositor

   Roger Davis

   Article

   Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

   Insert

   MKK7b1(K149A) (Map2k7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lys-149Ala

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

8. GFP-WIPI2d-TEVcs-TSF

   Plasmid

   #171420

   Purpose

   For the purifcation of GFP- WIPI2d protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. eNS2B47NS3 L30SF31S

   Plasmid

   #155319

   Purpose

   DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   L30 to S, F31 to S

   Promoter

   M13

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ADRA1A-Tango

    Plasmid

    #66213

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADRA1A (ADRA1A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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