We narrowed to 14,607 results for: pET

1. pUB-MSP1D1

   Plasmid

   #161609

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1 ( Q138W, L181W, Y236W)

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ825

   Plasmid

   #39315

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A)

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGD289

   Plasmid

   #228113

   Purpose

   pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Kva1 retron, ncRNA with 90 bp rpoB donor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ALIX-His(1-868, P801G)

   Plasmid

   #186808

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   ALIX-His (1-868, P801G) (PDCD6IP Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Proline 801 to Glycine

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTSA-13

   Plasmid

   #17327

   Depositor

   Takeshi Sano

   Article

   Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

   Insert

   streptavidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Truncated to include only amino acids 16-133 of m…

   Promoter

   Available since

   Feb. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. OmpT

   Plasmid

   #213137

   Purpose

   OmpT_native membrane expression

   Depositor

   Sebastian Hiller

   Article

   Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

   Insert

   OmpT
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKL002

   Plasmid

   #98921

   Purpose

   pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

   Depositor

   Joel Kralj

   Article

   Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

   Inserts

   PROPS
   GCaMP6f
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Atg24 1B

   Plasmid

   #197046

   Purpose

   His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

   Insert

   Atg24
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-Tev-WWP2

    Plasmid

    #186807

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-WWP2 (WWP2 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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