We narrowed to 13,184 results for: BASE
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Plasmid#101300PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.DepositorInsertalpha-Catenin
ExpressionMammalianMutationContains amino acids 1-509, 670-698, and 699-906 …Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUK21-NotI
Plasmid#51660PurposeBacterial cloning vector based on pUK21 (Kan)DepositorTypeEmpty backboneUseCloning vectorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUK21-deltaBB
Plasmid#51658PurposeBacterial cloning vector based on pUK21 (Kan)DepositorTypeEmpty backboneUseCloning vectorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-luxNeon
Plasmid#229947PurposeMammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)DepositorInsertluxNeon
ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-AKAR4
Plasmid#181957PurposeGenetically encoded FRET-based PKA activity reporter fused to AKAP79.DepositorInsertAKAP79-AKAR4 (AKAP5 Human)
TagsAKAP79, Cerulean, HIS tag, and cpVenus(E172)ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-BSCaM2
Plasmid#64855PurposeEndoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-BSCaM2
TagsCFP, N-terminal 27 amino acids from CYP450, and Y…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic_ME.1/ApoEpromoter
Plasmid#51435PurposeThis plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancerDepositorUseLuciferaseExpressionMammalianMutationME.1 enhancer is fused upstream of ApoE promoter …Available SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoCjCas9-BE4max-BlastR
Plasmid#194061PurposeMammalian expression of evoCjCas9 CBE4maxDepositorInsertevoCjCas9-BE4max
UseCRISPRExpressionMammalianAvailable SinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2Aneo
Plasmid#80032PurposeUsed to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trappingDepositorTypeEmpty backboneUseAAV and Cre/Lox; A platform vector to construct t…Available SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2224
Plasmid#67547PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197
Plasmid#67551PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
G12-CASE
Plasmid#190714PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagsNluc is inserted at A133/F134 within GNA12 and cp…ExpressionMammalianAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-F14F15S-Switch
Plasmid#99733PurposeFlp-based FLEX reporter (from RFP to GFP)DepositorInsertTagRFP and EGFP
ExpressionMammalianPromoterCAGAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-nlpE-gfp
Plasmid#253608PurposeExpresses a GFP-tagged NlpE protein for fluorescence-based visualizationDepositorInsertnlpE
TagsGFPExpressionBacterialAvailable SinceApril 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-cAMPr
Plasmid#160723PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.DepositorInsertS2-cAMPr
UseAAVExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN2AG5kwh
Plasmid#128253PurposeMammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.DepositorInsertBeta Globin Intron::hTetR::P2A::EGFP
UseSynthetic Biology; Flp-in expression vectorExpressionMammalianPromoterCMV D2ir promoterAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_11/pRSETB
Plasmid#51981Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nMDepositorInsertNano-lantern_CaM-4GS
Tags6xHisExpressionBacterialMutationS257G in Rluc8Available SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
ExpressionBacterialAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTPH405c
Plasmid#185726PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C) single PAM linker
UseCRISPRExpressionBacterialAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only