We narrowed to 9,707 results for: crispr plasmids

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pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14

Plasmid

#231365

Purpose

minBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-EGFP-W3SL_2x-U6-sasgAi14

Plasmid

#231366

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231364

Purpose

Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold

Plasmid

#226921

Purpose

Encoding SauriABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-GFPg1 (BB09)

Plasmid

#139461

Purpose

Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

GFP-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSCL.177

Plasmid

#184998

Purpose

Express -Eco1 EMX1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

EMX1 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.175

Plasmid

#184996

Purpose

Express -Eco1 HEK3 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

HEK3 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.180

Plasmid

#185001

Purpose

Express -Eco1 AAVS1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

AAVS1 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-CTRLg2

Plasmid

#139451

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_EBFP_Donor

Plasmid

#187455

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N EBFP donor (ATP1A1 Human)

Expression

Mammalian

Promoter

hPGK1

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg1 (CG509)

Plasmid

#139455

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-CTRLg1

Plasmid

#139450

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3_T142Q

Plasmid

#108110

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change Threonine 142 to Glutamine

Promoter

EFS promoter

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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PX458 BLTP3A knockout

Plasmid

#241256

Purpose

Knock-out plasmid targeting the second exon of human BLTP3A (UHRF1BP1)

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) KO gRNA (BLTP3A Human)

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1-13X-NOG(AtUBI10)-MTAP-LUC

Plasmid

#234371

Purpose

T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

NbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41

Use

Synthetic Biology

Tags

GB1, GP41, and sfGFP

Expression

Plant

Promoter

Arabidopsis Ubi10 and MTAP1 (synthetic)

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP103: pTarget(CyCAST)

Plasmid

#200849

Purpose

Target plasmid containing CyPSP1 and attachement site for CyCAST.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Inserts

PAM for CyCAST + CyPSP1
tRNA-Leu

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-EGFP-W3SL_BbsI(GGA)

Plasmid

#231362

Purpose

minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA

Plasmid

#233031

Purpose

To express a DjCas13d-compatible gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d-compatible gRNA expression cassette

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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