We narrowed to 17,265 results for: tes

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FLAG-KIF5A tail only

Plasmid

#166956

Purpose

Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A (820-1027aa)

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

Plasmid

#160616

Purpose

TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-H112A

Plasmid

#163619

Purpose

mouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Publication

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse SNX27-H112A (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

H112A

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBluescript II KS(+) EACMCV DNA-A 1.6mer

Plasmid

#159136

Purpose

Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 1998 DNA-A

Depositor

Vincent Fondong

Publication

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

EACMCV DNA-A 1.6mer

Use

Infectious clone for bombarding plants

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(800-868)

Plasmid

#141345

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).

Depositor

Lalit Deshmukh

Publication

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1His6-TEV-ALIX(800-868)

Tags

GB1-His6-TEV

Expression

Bacterial

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)

Plasmid

#156164

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence

Depositor

Kevin Park

Publication

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with addition of ER-retention sequence KDEL

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLCHKO_intergenic-intergenic_2

Plasmid

#155078

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

Depositor

Benjamin Blencowe, Jason Moffat

Publication

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pEAK-cKal7

Plasmid

#145801

Purpose

expresses Kalirin-7 with the c-front in mammalian cells

Depositor

Betty Eipper, Richard Mains

Publication

Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cKalirin-7 (Kalrn Rat)

Tags

none

Expression

Mammalian

Promoter

EIF2a

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHM123DEnv

Plasmid

#137668

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Publication

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR1

Plasmid

#136361

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

Depositor

Tatsuru Masuda

Publication

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-SMR2

Plasmid

#136362

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

Depositor

Tatsuru Masuda

Publication

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR1

Plasmid

#136359

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

Depositor

Tatsuru Masuda

Publication

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR2

Plasmid

#136360

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

Depositor

Tatsuru Masuda

Publication

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2Q2

Plasmid

#124668

Purpose

Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Publication

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

UBE2Q2 (UBE2Q2 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBA1

Plasmid

#104665

Purpose

Cloning Module

Depositor

Marco Trujillo

Publication

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBA1 (UBA1 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Craf SSDD

Plasmid

#118337

Purpose

Expression of human Craf

Depositor

Philip Stork

Publication

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Craf SSDD (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

SSDD

Promoter

CMV

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Craf R401H

Plasmid

#118334

Purpose

Expression of human Craf

Depositor

Philip Stork

Publication

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Craf R401H (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R401H

Promoter

CMV

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMX240

Plasmid

#23010

Depositor

Linda Wordeman

Publication

Wordeman et al J Cell Biol. 2007 Dec 3. 179(5):869-79.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCPBM

Tags

CenpB 1-167 and EGFP

Expression

Mammalian

Mutation

deleted MCAK amino acids 1-178, 584-718 mutated …

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Publication

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-Rac1 5'-UTR

Plasmid

#115354

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1

Depositor

Ming-Chih Lai

Publication

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rac1 5'-UTR (RAC1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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