We narrowed to 17,823 results for: Met;

1. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

   Plasmid

   #84483

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

   Plasmid

   #192785

   Purpose

   Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence, SNAPf-tag, and mXFPe

   Expression

   Mammalian

   Mutation

   mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #128587

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α (1.1 kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GEST-COMP-blac-flag-his

   Plasmid

   #111015

   Purpose

   Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

   Depositor

   Gavin Wright

   Article

   Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

   Insert

   gamete egress and sporozoite traversal protein (GEST) (PF3D7_1449000 )
   

   Use

   Tags

   Exogenous signal peptide of mouse origin and rat …

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

   Plasmid

   #192704

   Purpose

   Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence, SNAPf-tag, and meGFPm…

   Expression

   Mammalian

   Mutation

   meGFPm: gluatmic acid 142 to asparagine and histi…

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

   Plasmid

   #192784

   Purpose

   Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Ig k-chain leader sequence, SNAPf-tag, and mXFPm

   Expression

   Mammalian

   Mutation

   mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCF806-shABC2

   Plasmid

   #186714

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCF806-shABC1

   Plasmid

   #186713

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. G protein alpha-s-YFP

   Plasmid

   #55781

   Purpose

   This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   alpha-s-EE YFP (Gnas Rat)
   

   Use

   Tags

   EYFP flanked by SGGGS on each side was inserted i…

   Expression

   Mammalian

   Mutation

   Met was substituted for Gln69 in EYFP (Clontech).…

   Promoter

   CMV

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGLU_113

    Plasmid

    #225794

    Purpose

    RS421 library variant 13453

    Depositor

    Nili Ostrov

    Insert

    13453_[Tn5]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge