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pcDNA5/FRT/TO HIS DNAJA3

Plasmid

#19547

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)

Tags

His

Expression

Mammalian

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Publication

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-ER-ZapCY1

Plasmid

#36321

Depositor

Amy Palmer

Publication

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ER-ZapCY1 high affinity Zn(II) sensor

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pEntr-Tbx5flbio

Plasmid

#32968

Depositor

William Pu

Publication

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Tbx5 (TBX5 Human)

Use

Gateway Cloning

Tags

flag-bio

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAWp28

Plasmid

#73850

Purpose

pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Timothy Lu

Publication

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Type

Empty backbone

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pXL003-ishRNA-beta-catenin-2

Plasmid

#36299

Depositor

Sean Palecek

Publication

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-2 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pXL004-ishRNA-scramble

Plasmid

#36311

Depositor

Sean Palecek

Publication

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of scramble

Use

Lentiviral and RNAi

Tags

EF1a-TetR-IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available since

July 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSG 25

Plasmid

#63867

Purpose

Expression of beta-galactosidase at high levels in E. coli

Depositor

Albert Dahlberg

Publication

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MSCVhyg-F-Axin1

Plasmid

#24928

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Axin1 (AXIN1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

June 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
GAPDH

Plasmid

#155622

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

GAPDH (GAPDH Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-FLEX-BFP2-(mCherry)’

Plasmid

#115234

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

Depositor

Ian Wickersham

Publication

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Expression

Mammalian

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET32a-Spike-S-4P

Plasmid

#165094

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)

Expression

Bacterial

Available since

March 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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AdML

Plasmid

#11242

Depositor

Robin Reed

Publication

Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

AdML

Expression

Bacterial

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
3611 pcDNA3 Bim

Plasmid

#8804

Depositor

Stanley Korsmeyer

Publication

Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bim (Bcl2l11 Mouse)

Expression

Mammalian

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Publication

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Tags

myc

Expression

Bacterial

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV hSyn-fDIO-taCasp3-TEVp

Plasmid

#207997

Purpose

Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

Depositor

Kazunari Miyamichi

Publication

Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

Has service

AAV5

Insert

taCasp3-TEVp

Use

AAV

Promoter

hSyn promoter

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE

Plasmid

#198516

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination.

Depositor

Ofer Yizhar

Publication

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has service

AAV1 and AAV5

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137139

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP

Plasmid

#137153

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Foff 2.0-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

W179F

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-iC++-EYFP

Plasmid

#137157

Purpose

Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Coff/Fon-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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