We narrowed to 9,648 results for: Ada;

1. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

   Plasmid

   #123616

   Purpose

   CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   NLS-nCas9-NLS-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

   Plasmid

   #111387

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

   Plasmid

   #178827

   Purpose

   Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

   Depositor

   Adam Cohen

   Article

   Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

   Insert

   SomQuasAr6a_EGFP-P2A-somCheRiff_HA
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP, HA

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Clover-LaminA-C-18

   Plasmid

   #56370

   Purpose

   Localization: Nuclear Envelope, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pZE21/UBP1/ClpS

   Plasmid

   #98566

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   ATP-dependent Clp protease adapter protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   In a synthetic operon downstream of UBP1 and Remo…

   Promoter

   Same as UBP1 (operon) and Tet promoter (aTc induc…

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBGE1.0

   Plasmid

   #165986

   Purpose

   Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.

   Depositor

   Bogumil Karas

   Article

   Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

   Inserts

   RK2/RP4 origin of transfer
   repA1B1C1
   nourseothricin N-acetyl transferase
   spectinomycin/streptomycin resistance
   

   Use

   Synthetic Biology; Bacterial and yeast cloning; c…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

   Plasmid

   #157951

   Purpose

   Lentiviral expression of HBG site 1 sgRNA

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-pro-siRNA-V2-LMNA

   Plasmid

   #111088

   Purpose

   pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

   Depositor

   Linfeng HUANG

   Article

   Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

   Insert

   Lamin A/C (LMNA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLPC-L647RLaminA-CS

   Plasmid

   #69062

   Purpose

   encodes a lamin A mutant with Leucine 647 mutated to an Arginin and with a substitution of cysteine in CaaX-motif by a serine

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   LaminA (LMNA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Lamin A with Leucine 647 mutated to Arginine and …

   Promoter

   CMV

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pAP1892-1

    Plasmid

    #105247

    Purpose

    human GFP11 with tagRFP 33bp downstream in Lamin A/C

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAP1716-1

    Plasmid

    #105245

    Purpose

    human eGFP with homology arms for Lamin A/C

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    eGFP with homology arms for Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FKBP-beta2(616-951)-GFP Double

    Plasmid

    #100729

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif and …

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP-beta2(616-951)-GFP ∆CBM

    Plasmid

    #100728

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif

    Promoter

    CMV

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-VRERRA-P2A-GFP-PGK-Puro

    Plasmid

    #110865

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FKBP-beta2(616-951)-GFP Y215A

    Plasmid

    #100727

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform Y215A mutation (Y815A in full length)

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-LacI-Cyclin B1 ND

    Plasmid

    #234706

    Purpose

    allows tethering of non degradable (ND) cyclin B1 protein to lacO repeats in specific chromatin loci

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAGE1.0

    Plasmid

    #165983

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-Puro-sgBRSK2

    Plasmid

    #138687

    Purpose

    Expresses a human BRSK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgBRSK2 human (BRSK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-VRER-P2A-GFP-PGK-Puro

    Plasmid

    #110862

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge