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hBCAM-M4

Plasmid

#203320

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hBCAM/hMUC18

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-C2 of hMuc18 fused to Ig like C3 of hBCAM

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-M2

Plasmid

#203318

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hBCAM/hMUC18

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-V2 of hMuc18 fused to Ig like C1-C3 of hB…

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-M1

Plasmid

#203317

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hBCAM/hMUC18

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1 of hMuc18 fused to Ig like V2-C3 of hBCAM

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-B4

Plasmid

#203316

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hMUC18/hBCAM

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-C2 of hBCAM fused to Ig like C3 of hMUC18

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-B3

Plasmid

#203315

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hMUC18/hBCAM

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-C1 of hBCAM fused to Ig like C2-C3 of hMUC…

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-B1

Plasmid

#203313

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hMUC18/hBCAM

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1 of hBCAM fused to Ig like V2-C3 of hMUC18

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-M3

Plasmid

#203319

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hBCAM/hMUC18

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-C1 of hMuc18 fused to Ig like C2-C3 of hBC…

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-B2

Plasmid

#203314

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hMUC18/hBCAM

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-V2 of hBCAM fused to Ig like C1-C3 of hMU…

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.A02R

Plasmid

#198658

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with FL protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.A02L

Plasmid

#198657

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with BF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.A01R

Plasmid

#198656

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.A01L

Plasmid

#198655

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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DsRed -7e

Plasmid

#191765

Purpose

Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of negatively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-CR-PA

Plasmid

#175445

Purpose

HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Tags

BiP Secretion Peptide and Thrombin-Protein A

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide

Plasmid

#121993

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide#2

Plasmid

#121994

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-opa1 guide

Plasmid

#121995

Purpose

An entry vector with U6a and U6c promoter driving opa1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

opa1 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S + STS:P4S

Plasmid

#139789

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

biosynthetic enzymes 4CL and STS in fusion with coiled-coil segments

Expression

Bacterial

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-hsg(G:H)

Plasmid

#138259

Purpose

Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mCherry targeting sgRNAs G and H

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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