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pABE-dGFP-gRNA

Plasmid

#226864

Purpose

Human gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEs

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

Sp-gRNA

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFSW FLEx mCherry-KASH

Plasmid

#139652

Purpose

Conditional expression of mCherry-KASH

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry-KASH

Expression

Mammalian

Promoter

Synapsin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ154

Plasmid

#69305

Purpose

Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9 (Arabidopsis codon optimized)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Nat

Plasmid

#232097

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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TL1 DdCBE A1 (DddA6-C)

Plasmid

#221992

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: T1413I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TL1 DdCBE T2 (DddA6-N)

Plasmid

#221993

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: Q1310R, S1330I, T1380I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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LbdCas12a VPR

Plasmid

#188513

Purpose

Expresses FLAG tagged LbdCas12a VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D832A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG

Plasmid

#79877

Purpose

FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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P2A-NLS-tdtomato-EPG backbone

Plasmid

#215548

Purpose

An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Type

Empty backbone

Use

CRISPR

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

Plasmid

#61255

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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