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pT-GFP-rG2

Plasmid

#188968

Purpose

IPTG inducible GFP with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

GFP
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-AtpU6-29

Plasmid

#160219

Purpose

AtpU6-29 Golden Gate level 0 piece to express gRNAs

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtU6-29 promoter

Use

Golden gate level 0 piece to express grnas

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9341 (pgRNA_IX-1_NatMX)

Plasmid

#161593

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9343 (pgRNA_XV-1_NatMX)

Plasmid

#161595

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9358(VII-1_Markerfree_BackBone)

Plasmid

#161599

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9359(VIII-1_Markerfree_BackBone)

Plasmid

#161600

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9361(XIII-1_Markerfree_BackBone)

Plasmid

#161602

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9363(XVI-1_Markerfree_BackBone)

Plasmid

#161604

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmDCP2-dsRNAres-V5His6_I

Plasmid

#146584

Purpose

Insect Expression of DmDCP2-dsRNAres

Depositor

Elisa Izaurralde

Article

Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

Insert

DmDCP2-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP2-dsRNAres-V5His6_I

Plasmid

#146585

Purpose

Insect Expression of DmDCP2-dsRNAres

Depositor

Elisa Izaurralde

Article

Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

Insert

DmDCP2-dsRNAres (DCP2 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-SBP-DmDCP1-dsRNAres_J

Plasmid

#146711

Purpose

Insect Expression of DmDCP1-dsRNAres

Depositor

Elisa Izaurralde

Article

Nishihara et al Nucleic Acids Res. 2013 Oct;41(18):8692-

Insert

DmDCP1-dsRNAres (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179010

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179009

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF_340-368-GST-V5His6_AH

Plasmid

#148935

Purpose

Insect Expression of DmGIGYF_340-368

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_340-368 (Gyf Fly)

Expression

Insect

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1

Plasmid

#183404

Purpose

Dual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-GID-SmBiT-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1

Plasmid

#183399

Purpose

Dual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-NanoLuc-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-1666_T

Plasmid

#147580

Purpose

Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-58-DmeIF4Ga_687-1666 (4E-T Fly)

Expression

Insect

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)

Plasmid

#179080

Purpose

Parent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminators

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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