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DJ-L1 gRNA #3

Plasmid

#247543

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #1

Plasmid

#247542

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1751

Plasmid

#246066

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 ori

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Panoptes locus

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-PUM2

Plasmid

#245723

Purpose

Express Flag-HA-PUM2 in mammalian cells

Depositor

Joshua Mendell

Article

Thomas et al Genes Dev. 2022 May 1;36(9-10):550-565.

Insert

Flag-HA-PUM2 (PUM2 Human)

Use

Lentiviral

Tags

Flag-HA

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1302-Pro35S:Irp9

Plasmid

#246319

Purpose

Overexpression of a bacterial bifunctional salicylate synthase targeted to the cytosol

Depositor

Chung-Jui Tsai

Article

Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

Insert

Irp9

Expression

Plant

Promoter

cauliflower mosaic virus 35S

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1302-Pro35S:Fd-Irp9

Plasmid

#246318

Purpose

Overexpression of a bacterial bifunctional salicylate synthase, Irp9, targeted to the chloroplast

Depositor

Chung-Jui Tsai

Article

Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

Insert

Fd-Irp9

Expression

Plant

Promoter

cauliflower mosaic virus 35S

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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EK0574 CAG-ADAR1-p150

Plasmid

#247268

Purpose

Expresses human ADAR1-p150 from the CAG promoter

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

ADAR1-p150 (ADAR Human)

Expression

Mammalian

Mutation

WT

Promoter

CAG

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK250 CMV-TO-SCN1A (exons 20, 20N, 21)

Plasmid

#247285

Purpose

Expresses human SCN1a exons 20, 20N, 21

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

SCN1a exons 20, 20N, 21 (SCN1A Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1729

Plasmid

#246063

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Panoptes locus

Expression

Bacterial

Promoter

pTet promoter

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R4A,R5A)

Plasmid

#245221

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

R4A,R5A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AtHKT1_pYES2

Plasmid

#244550

Purpose

High- copy episomal vector for galactose-inducible expression of AtHKT1 protein in Saccharomyces cerevisiae.

Depositor

Julian Schroeder

Article

Uozumi et al Plant Physiol. 2000 Apr;122(4):1249-59.

Insert

AtHKT1 (HKT1 Mustard Weed)

Expression

Yeast

Promoter

T7 promoter

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

Plasmid

#244844

Purpose

Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

iCas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pZNp-2

Plasmid

#245373

Purpose

Negative control, firefly luciferase mutated

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZ_pF-3

Plasmid

#245374

Purpose

Negative control, NanoLuciferase mutated

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆7

Plasmid

#245367

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆7 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

7-bp deletion, H96Vfs*29

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆8

Plasmid

#245368

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆8 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

synthetic 8-bp deletion

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-wt

Plasmid

#245364

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-wt (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆59

Plasmid

#245365

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆59 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

59-bp deletion, D78Vfs*6

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆11

Plasmid

#245366

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆11 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-CpG-free-mStayGold

Plasmid

#241386

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CpG-free promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CpG-free

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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