We narrowed to 53,429 results for: plasmid

1. pGBW-m4046805

   Plasmid

   #145589

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4046935

   Plasmid

   #145580

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4046725

   Plasmid

   #145581

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046761

   Plasmid

   #145582

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4046777

   Plasmid

   #145583

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGBW-m4046734

   Plasmid

   #145576

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046826

   Plasmid

   #145687

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. YSBE415: pTOP_ACA1_Vc_HOG1 terminator

   Plasmid

   #122434

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   C-terminal fragment of Venus protein
   

   Use

   Tagging proteins in cryptococcus neoformans

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. YSBE416: pTOP_CAC1_Vn_HOG1 terminator

   Plasmid

   #122435

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   N-terminal fragment of Venus protein
   

   Use

   Tagging proteins in cryptococcus neoformans

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pATF1-donor

    Plasmid

    #112328

    Purpose

    CRISPR donor plasmid to tag human transcription factor ATF1 with GFP

    Depositor

    Kevin White

    Insert

    ATF1 homology arms flanking EGFP-IRES-Neo cassette (ATF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:12:50819876:T>A, hg38:12:50819888:T>C,…

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LI F-G Gent

    Plasmid

    #104522

    Purpose

    LI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Apramycin resistance gene
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p236_LTJ_sgRNAFoxp3sf/J

    Plasmid

    #82676

    Purpose

    sgRNA targeting murine Foxp3 scurfy. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting Foxp3 sf/J
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. DMTF1

    Plasmid

    #36895

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DMTF1 (DMTF1 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains Myb-like 1 domain, residues 220-274

    Promoter

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. FUW-TetON-GFP

    Plasmid

    #115495

    Purpose

    Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUDP025

    Plasmid

    #103874

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDP082

    Plasmid

    #103875

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUDP013

    Plasmid

    #103873

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167849

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pSc1

    Plasmid

    #80436

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR P2R-P3-IRES_ECFP

    Plasmid

    #48354

    Purpose

    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    IRES_ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    ECFP translation is mediated by an IRES. Contains…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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