We narrowed to 10,162 results for: Coli
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Plasmid#221326PurposeDox-inducible expression of Tir in mammalian cells by retroviral transductionDepositorInsertTir
UseRetroviralTagsHN-FLAGPromoterTRE3GVAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR88
Plasmid#203422Purposeempty BLINCAR plasmid for payload delivery, no payload, no genomic targetDepositorInsertsCAR copy 1
P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
CAR copy 2
ExpressionYeastAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA337
Plasmid#214750PurposeMedium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validationDepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
ExpressionBacterialAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-TrxF
Plasmid#207454PurposeE. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tagDepositorInsertThioredoxin F
Tags6xHisExpressionBacterialMutationCodon optimized for E coli expressionPromoterT7Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PGUS
Plasmid#207827PurposeFLAG-tagged PGUS driven by CMV promoterDepositorInsertPGUS
UseLentiviralTagsFLAGPromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3
Plasmid#201534PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB3
UseSynthetic BiologyMutationrhaS W190GPromoterPrhaBAD (rhamnose)Available SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-GUS
Plasmid#202137PurposeLevel 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct.DepositorInsertBeta glucuronidase gene
UseSynthetic BiologyMutationNoneAvailable SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYG215
Plasmid#200837PurposeTranscribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.DepositorInsertglmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsGFPExpressionBacterialPromoterP-LlacO-1Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C1
Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptAvailable SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2
Plasmid#201533PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB2
UseSynthetic BiologyMutationrhaR L128+1bp (frameshift), ilvA G455CPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only