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pPD159 dsRedExpress2-ZFi43

Plasmid

#138799

Purpose

Constitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

dsRedExpress2-ZFi43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG216

Plasmid

#114002

Purpose

Level 2 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242A

Plasmid

#32823

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242A

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pPOTv7-blast-eGFP

Plasmid

#179781

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

eGFP

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL50005

Plasmid

#117536

Purpose

Level0 Golden Gate part: c-terminal tag, YFP

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

c-term tag YFP

Use

Synthetic Biology

Mutation

G65S, L68V, A72S, Y203T, L231H

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pB6N_RB38-Mt-tk_v8_AGKS

Plasmid

#191514

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE RB38-Mt-tk AGKS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB7N_LT51-Mt-tk_v8_AGKS

Plasmid

#191515

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE LT51-Mt-tk AGKS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC

Plasmid

#148443

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-Malat

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOGG004

Plasmid

#113979

Purpose

Vector construction module. Level 1 golden gate cloning site module with LacZ reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Level 1 golden gate cloning site

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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