159,258 results

1. GFP-TPD54

   Plasmid

   #172448

   Purpose

   Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

   Insert

   TPD54 (TPD52L2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L4440_BioBrick-hsf-1-sgRNA-B

   Plasmid

   #177787

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hsf-1 (hsf-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pInducer20 ALPK1 Y254C

   Plasmid

   #231587

   Purpose

   ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

   Depositor

   Thomas Henry

   Article

   Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

   Insert

   ALPK1 (ALPK1 Human)
   

   Use

   Lentiviral

   Tags

   3X flag

   Expression

   Mammalian

   Mutation

   changed tyrosine 254 to cysteine

   Promoter

   doxycycline

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIMAY

   Plasmid

   #68939

   Purpose

   E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

   Depositor

   Tim Foster

   Article

   Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

   Type

   Empty backbone

   Use

   Staphylococcal allelic exchange vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJBM4

   Plasmid

   #225291

   Purpose

   E. coli-Campylobacter shuttle vector; TetR

   Depositor

   Robert Moore

   Article

   McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

   Insert

   Tet(O) and its putative promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC

   Plasmid

   #221363

   Purpose

   Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC; to be used with Anti-His [IPI-HAP1/5.13.22] light chain (Plasmid 221364) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa

   Plasmid

   #221364

   Purpose

   Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa; to be used with Anti-His [IPI-HAP1/5.13.22] heavy chain (Plasmid 221363) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. TFORF3132

   Plasmid

   #144608

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ESR2 (ESR2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R777-E245 Hs.RASGRP2

    Plasmid

    #70529

    Purpose

    Gateway ORF clone of human RASGRP2 [NM_153819.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASGRP2 (RASGRP2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge