We narrowed to 19,197 results for: gateway

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pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163073

Purpose

Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163076

Purpose

Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6-AMPK alpha1 (1-312)

Plasmid

#162131

Purpose

Expression vector for constitutively active AMPK alpha 1

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

Insert

AMPK alpha1 (1-312) (PRKAA1 Rat)

Use

Lentiviral

Tags

V5

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Plasmid

#159808

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLX302 MX1-V5 puro

Plasmid

#158640

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged MX1.

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

Mx1 (MX1 Human)

Use

Lentiviral

Tags

V5

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_empty

Plasmid

#160093

Purpose

Control lentiviral vector for mammalian gene expression

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1

Plasmid

#159576

Purpose

Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)

Tags

His6

Expression

Insect

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAct-PE2-HygroR

Plasmid

#149552

Purpose

Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_3xflag_NRF2(RR)

Plasmid

#136535

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Mutation

This plasmid is developed by mutating 3 base pair…

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK_neo_3xflag_CHEK2

Plasmid

#136536

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged CHEK2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

CHEK2 (CHEK2 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Available Since

June 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

Plasmid

#153008

Purpose

Expresses glucagon peptide on mammalian cell surface

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

Glucagon-ICAM1-pre-mGRASP

Tags

3x HA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-Y530F

Plasmid

#140319

Purpose

Expresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Y530F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2-CreLite

Plasmid

#131783

Purpose

Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC, PIF6CreN, and mTagBFP2

Use

Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

Tags

mTagBFP2

Expression

Mammalian

Promoter

Ubi

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-GABARAPL1 WT

Plasmid

#123117

Purpose

Expresses EGFP-GABARAPL1 wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-GABARAPL2-GFP

Plasmid

#123113

Purpose

Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-GUCY2C

Plasmid

#116749

Purpose

Lentiviral expression of GUCY2C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GUCY2C (GUCY2C Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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