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Showing: 9541 - 9560 of 65834 results

  2. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  8. Flag-neo-PP1-Beta

    Plasmid
    #171269
    Purpose
    Mammalian expression of 3x-Flag-tagged human PP1 beta catalytic subunit
    Depositor
    Steven Balk
    Insert
    3x-Flag-tagged human PP1 beta catalytic subunit (PPP1CB Human)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171269-plasmid-map-sequence-id-335788

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  10. Tubulin-mGreenLantern

    Plasmid
    #164460
    Purpose
    Localization of mGreenLantern fluorescent protein to tubulin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    Tubulin
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164460-plasmid-map-sequence-id-322770

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  11. pSicoR Dnmt1

    Plasmid
    #12166
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF Dnmt1 shRNA expression.
    Depositor
    Tyler Jacks
    Publication
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Insert
    Dnmt1 shRNA (Dnmt1 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, …
    Available since
    June 30, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 9541 - 9560 of 65834 results