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pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP

Plasmid

#137148

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

Con/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.A184S

Plasmid

#106192

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has service

AAV1

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_BiVIPe4_dTomato_nlsdTomato

Plasmid

#213855

Purpose

AAV construct expressing dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_dTomato_nlsdTomato

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7a1

Plasmid

#14538

Depositor

Roger Davis

Publication

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1 (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pBABE MDER

Plasmid

#13494

Depositor

Stephen Tapscott

Publication

Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Mouse, Human)

Use

Retroviral

Expression

Mammalian

Mutation

full length MyoD was fused to aa282-595 of Esr1. …

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCrx-DsRed

Plasmid

#13765

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Crx promoter (Crx Mouse)

Tags

DsRed2

Expression

Mammalian

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP3

Plasmid

#59038

Purpose

for baculovirus expression of mouse CAMSAP3 in Sf9 cells

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 (Camsap3 Mouse)

Tags

His and eGFP

Expression

Insect

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pComb8

Plasmid

#63889

Purpose

For phage display of antibody libraries by fusion to modified geneVIII.

Depositor

Carlos Barbas

Publication

Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

Type

Empty backbone

Use

Phage display

Tags

geneVIII

Expression

Bacterial

Promoter

lacZ

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pENTR223-PRDX5K85R

Plasmid

#98694

Purpose

Gateway cloning of human PRDX5-K85R

Depositor

Mohan Babu

Publication

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway Cloning

Mutation

K85R

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5K83R

Plasmid

#98693

Purpose

Gateway cloning of human PRDX5-K83R

Depositor

Mohan Babu

Publication

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway Cloning

Mutation

K83R

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5E80K

Plasmid

#98692

Purpose

Gateway cloning of human PRDX5-E80K

Depositor

Mohan Babu

Publication

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway Cloning

Mutation

E80K

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTH-PanCen-mVenus

Plasmid

#49640

Purpose

TALEColor-mVenus-Pan-Centromere

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Type

Empty backbone

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-Neo-Bam E2F6

Plasmid

#37965

Depositor

Jacqueline Lees

Publication

Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E2F6 (E2F6 Human)

Expression

Mammalian

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_BiSSTe4_ChR2_mCherry

Plasmid

#213945

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_ChR2_mCherry

Depositor

Gordon Fishell

Publication

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has service

AAV1 and AAV PHP.eB

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

Plasmid

#110057

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Publication

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Per2

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Y701F-STAT1

Plasmid

#71453

Purpose

Lentiviral expression of Y701F-STAT1

Depositor

George Stark

Publication

Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

FLAG

Mutation

Y701F

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET32a-HD16Q

Plasmid

#11487

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 16 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 16 glutamines, vector m…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MGP M155

Plasmid

#26527

Depositor

David Baltimore

Publication

O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

Insert

miR-155 (Mir155 Mouse)

Use

Retroviral

Tags

GFP

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-AU1-mTOR-L1460P

Plasmid

#19995

Depositor

Fuyuhiko Tamanoi

Publication

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian target of rapamycin-L1460P (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

Changed Leucine 1460 to Proline

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCW-codon optimized catalytically inactive PHGDH

Plasmid

#154903

Purpose

Expresses codon optimized catalytically inactive PHGDH in mammalian cells

Depositor

Michael Pacold

Publication

Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Three mutations: D175N, R236K, H283A

Promoter

TRE

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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