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gh144

Plasmid

#106803

Purpose

expression of gRNA targeting LARGE

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

LARGE

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh119

Plasmid

#106789

Purpose

expression of gRNA targeting GCNT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT6

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh20

Plasmid

#106704

Purpose

expression of gRNA targeting GALNT20

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT20

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBac(3xP3-gTc’v; Valcyrie.LR)

Plasmid

#119066

Purpose

Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)

Depositor

Michalis Averof

Article

Gilles et al Development. 2019 Jan 4;146(1). pii: dev

Inserts

pBacL
Tc-U6a-Lox.L
Tc-hsp68 Promoter
NLS-Cas9
Tc-hsp68 3'UTR
Tc-U6B-Lox.R
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR

Expression

Insect

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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gh163

Plasmid

#106816

Purpose

expression of gRNA targeting TMTC4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

TMTC4 (TMTC4 Human)

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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gh41

Plasmid

#106715

Purpose

expression of gRNA targeting ST6GAL2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GAL2 (ST6GAL2 Human)

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMAZ.1.0-gDNA

Plasmid

#112455

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MAZ

Depositor

Kevin White

Insert

MAZ (MAZ Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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gh156

Plasmid

#106823

Purpose

expression of gRNA targeting GYLT1B (LARGE2)

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GYLT1B (LARGE2)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mRuby-N

Plasmid

#113752

Purpose

Gateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2

Use

CRISPR; Gateway entry vector

Tags

mRuby2

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1323

Plasmid

#111125

Purpose

Expresses non-targeting_01224 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

Non-targeting gRNA

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmEGFP-N

Plasmid

#113747

Purpose

Gateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

2xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmEGFP-N

Plasmid

#113748

Purpose

Gateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

3xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmEGFP-C

Plasmid

#113750

Purpose

Gateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

2xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmRuby-N

Plasmid

#113753

Purpose

Gateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

2xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-2xmRuby-C

Plasmid

#113756

Purpose

Gateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

2xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65(100-261)-RTA(75-190)

Plasmid

#99672

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

VP64-p65(101-261)-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF511

Plasmid

#104037

Purpose

Donor vector for 3' FLAG tag of human ZNF511

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF511 homology arms (ZNF511 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K310A

Plasmid

#91856

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K310A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K310A

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_D1078A

Plasmid

#91860

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) D1078A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

D1078A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_N314A

Plasmid

#91858

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) N314A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

N314A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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