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BCJJ223

Plasmid

#117509

Purpose

AtMGE1 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pMGE1:AATG:BsaI

Expression

Plant

Promoter

AtMGE1

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL12021

Plasmid

#117511

Purpose

AtICU2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pICU2:AATG:BsaI

Expression

Plant

Promoter

AtICU2

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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FISSEQ-hgRNA-Design1

Plasmid

#100571

Purpose

FISSEQ-hgRNA-Design1 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design1

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NG

Plasmid

#90420

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NG

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NN

Plasmid

#90419

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NN

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_fcpA_NI

Plasmid

#90418

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NI

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM9_NR_NI

Plasmid

#90422

Purpose

TALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NI

Depositor

Peter Kroth

Article

Serif et al Algal Research Volume 23, April 2017, Pa

Insert

TALEN Backbone

Use

Phaeodactylum tricornutum

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 upsilon

Plasmid

#44932

Depositor

Robert Ferl

Article

Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

Insert

14-3-3 upsilon (GRF5 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pCri-7a

Plasmid

#61316

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST17_A1LigN_EC

Plasmid

#22296

Depositor

Oscar Millet

Article

Tadeo et al PLoS Biol. 2009 Dec;7(12):e1000257. doi:

Insert

A1 domain NAD+ dependent DNA ligase N

Tags

His tag

Expression

Bacterial

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only
pAL750 :: msfGFP

Plasmid

#250661

Purpose

Inducible promoter Pxyl expressing msfGFP in haloarcheon Haloferax volcanii

Depositor

Alexandre Bisson

Article

Rados et al Front Microbiol. 2023 Aug 10;14:1204876.

Insert

Monomeric Superfolder Green Fluorescent Protein

Use

Haloarchaeal expression

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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POC1551

Plasmid

#226498

Purpose

pMOBK360_StandVKL: Standard Vector (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1552

Plasmid

#226499

Purpose

pMOBC360_StandVC: Standard Vector (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1423

Plasmid

#221559

Purpose

Plasmid designed to assay the site-selective methylation protection approach using the non-switchable methylases

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE mTFP1-actin

Plasmid

#229970

Purpose

encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mTFP1-actin

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE H2B iRFP720

Plasmid

#229968

Purpose

encodes a H2B-iRFP720 fusion contruct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

H2B iRFP720

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE mito-mCherry

Plasmid

#229966

Purpose

encodes a mCherry targeted to mitochondria under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mito-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB EF1alpha

Plasmid

#229954

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE GTS-mTagBFP

Plasmid

#229969

Purpose

encodes a mTagBFP targeted to Golgi apparatus under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

GTS-mTagBFP

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE EYFP-tubulin

Plasmid

#229967

Purpose

encodes a YFP-tubulin fusion contruct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

EYFP-tubulin

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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