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pTRK669

Plasmid

#71313

Purpose

Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry Paxillin

Plasmid

#50526

Depositor

Kenneth Yamada

Insert

PXN paxillin (PXN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

Plasmid

#205565

Purpose

Express human NEDD4L (delta C2 isoform)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

NEDD4L (NEDD4L Human)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

delta C2 isoform

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

Plasmid

#224006

Purpose

AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

minBG

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti.CAG.H2B-Dendra2.W

Plasmid

#51005

Purpose

Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

Depositor

Rusty Lansford

Insert

H2B-Dendra2

Use

Lentiviral

Tags

histone2B

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G

Plasmid

#177960

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PRB

Plasmid

#89130

Purpose

mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-B (PGR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTAT-HA

Plasmid

#35612

Depositor

Steven Dowdy

Article

Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

Type

Empty backbone

Use

Tags

6xHIS, HA, and TAT

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

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p5E-hPGK

Plasmid

#82579

Purpose

5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

Human PGK promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAB46 (CK2beta)

Plasmid

#27085

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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