We narrowed to 9,980 results for: Uty

1. pcDNA3-ALK6 Q203D

   Plasmid

   #80883

   Purpose

   mammalian expression of ALK6 Q203D

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK6 (Bmpr1b Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q203D (constitutively active)

   Promoter

   CMV

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pScalps_Puro_mTet2 catalytic domain HxD

   Plasmid

   #79611

   Purpose

   Expression of catalytically inactive mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Mutation

   Mutant Tet2 with H1302Y, D1304A substitutions in …

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1-FLT3-D835N-V5/HIS

   Plasmid

   #236008

   Purpose

   expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   D835N substitution

   Promoter

   CMV

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDule2-3-nitroTyrosine (A7)

   Plasmid

   #174079

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Expression

   Bacterial

   Mutation

   Y32H H70T D158H I159A L162R

   Promoter

   GlnRS (constitutive)

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xF2X-P2A-Puro

   Plasmid

   #110873

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS-2X(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

   Plasmid

   #208038

   Purpose

   Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   PML (isoform IVa) (PML Human)
   

   Use

   Lentiviral

   Tags

   FLAG, N-terminal Split-TurboID

   Promoter

   EFS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEN6-Ubc5 Sc

   Plasmid

   #83234

   Purpose

   pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Inserts

   Ubiqutin (UBC Human)
   Ubc 5 (UBC5 Budding Yeast)
   Uba 1
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDHa_mDDX5_FL_wt

   Plasmid

   #88869

   Purpose

   constitutive expression of Ha-DDX5 in mammalian cells

   Depositor

   Dan Littman

   Article

   Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

   Insert

   DDX5 (Ddx5 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER22-MKK6E-Flag

   Plasmid

   #99259

   Purpose

   Lentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.

   Depositor

   Peter Vincent, Alejandro Adam

   Article

   Adam et al PLoS One. 2016 Sep 7;11(9):e0161975. doi

   Insert

   MKK6E (MAP2K6 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S207E T211E

   Promoter

   TRE2

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-mGFAP(ABC1D)-2pabPAC(F198Y)

    Plasmid

    #234548

    Purpose

    to express a version with reduced constitutive activity (thanks to point mutation F198Y) of the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

    Depositor

    Hajime Hirase

    Article

    Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

    Insert

    2pabPAC(F198Y)
    

    Use

    AAV

    Mutation

    F198Y

    Promoter

    mGFAP(ABC1D)

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid

    #89463

    Purpose

    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Tags

    E. coli DHFRdd destabilization domain and mCherry…

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    TRE3G

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNoROS-CAT

    Plasmid

    #170152

    Purpose

    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation

    Depositor

    Dimitri Pestov

    Article

    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.

    Inserts

    catalase (Cat Mouse)
    roGFP2
    

    Use

    Genome integration via sleeping beauty transposon…

    Tags

    FLAG tag and Nucleolar Localization Sequence (NoL…

    Expression

    Mammalian

    Promoter

    RPBSA and TRE promoter

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid

    #99277

    Purpose

    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mOrange2-IRES-EGFP
    Puro
    

    Use

    Lentiviral

    Tags

    EGFP, H2B, IRES, PGK, and mOrange2

    Promoter

    EF1 alpha-human and PGK-mouse

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCXN2KS-Dsup

    Plasmid

    #90019

    Purpose

    To establish stable line expressing Dsup in constitutive manner

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRHR1-DuET

    Plasmid

    #213215

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CRHR1 (CRHR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDHa_mDDX5_FL_K144N

    Plasmid

    #88870

    Purpose

    constitutive expression of Ha-DDX5 K144N in mammalian cells

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (Ddx5 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K144N

    Promoter

    CMV

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LPAR1-DuET

    Plasmid

    #213329

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    LPAR1 (LPAR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDule-3-nitroTyrosine (A7)

    Plasmid

    #174078

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCEP4-FLAG-TAPBPR-TM-VC

    Plasmid

    #135499

    Purpose

    Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Tags

    Luminal/Extracellular FLAG epitope tag, Signal pe…

    Expression

    Mammalian

    Mutation

    switched transmembrane domain with that of MHC-I

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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