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Showing: 1881 - 1920 of 2191 results

  1. B52_puro_gRNA_3418711_KCNB2

    Plasmid
    #197558
    Purpose
    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)
    Depositor
    Darius Bägli
    Insert
    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    Use
    Grna with puromycin selection
    Tags
    puromycin resistance cassette under CMV promotion
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197558-plasmid-map-sequence-id-388709

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  2. RCav3.1/pcDNA3-HE3

    Plasmid
    #197324
    Purpose
    Plasmid expressing an antigen targeting the Cav3.1 calcium channel subunit
    Depositor
    Edward Perez-Reyes
    Insert
    Cav3.1 (Cacna1g Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197324-plasmid-map-sequence-id-388203

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  3. GIT1-Flag/pBK

    Plasmid
    #197313
    Purpose
    Plasmid expressing an antigen targeting GIT1
    Depositor
    Eunjoon Kim
    Insert
    GIT1 (Git1 Rat)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197313-plasmid-map-sequence-id-388148

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  4. pGADT7-AP-2 σ2

    Plasmid
    #198345
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-2 σ2
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    R42G substitution, and silent substitutions in co…
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198345-plasmid-map-sequence-id-387832

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  18. PVALB mAb_BCN14.1.1C9_LC_Rat Ig Kappa

    Plasmid
    #182891
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182891-plasmid-map-sequence-id-360625

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  19. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid
    #182894
    Purpose
    Mammalian expression of PVALB rat mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) heavy chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182894-plasmid-map-sequence-id-360571

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  20. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid
    #182895
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182895-plasmid-map-sequence-id-360643

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  21. mCherry-7aa-cb5-pEGFP-C1

    Plasmid
    #177407
    Purpose
    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177407-plasmid-map-sequence-id-348154

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  22. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid
    #190174
    Purpose
    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway
    Depositor
    Gary Rudnick
    Article
    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.
    Insert
    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    Use
    Tags
    FLAG, His6, and c-myc
    Expression
    Mammalian
    Mutation
    changed Cys109 to alanine, changed tyr107 to cyst…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190174-plasmid-map-sequence-id-377202

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  27. RAT +D122H

    Plasmid
    #187426
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al bioRxiv 2021.11.29.470343
    Inserts
    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    Use
    Tags
    Expression
    Insect
    Mutation
    changed aspartic acid at 129 for histidine
    Promoter
    PH and p10
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    187426-plasmid-map-sequence-id-374214

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  28. RAT +R111E

    Plasmid
    #187425
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al bioRxiv 2021.11.29.470343
    Inserts
    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    Use
    Tags
    Expression
    Insect
    Mutation
    changed arginine at 118 to glutamic acid
    Promoter
    PH and p10
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    187425-plasmid-map-sequence-id-374210

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  40. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

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Showing: 1881 - 1920 of 2191 results