Addgene

Showing: 941 - 960 of 1042 results

Results per page:

Sort results by:

miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

Plasmid

#167820

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE-EGFP-FLAG-TK1

Plasmid

#161919

Purpose

Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Thymidine kinase, cytosolic (TK1 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
miniTol2 x EF1a_EKAREN4 + PGK-puro

Plasmid

#167824

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV x EF1a_EKAREN4(TA)-ires-puro

Plasmid

#167830

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

Plasmid

#167826

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
miniTol2 x EF1a_EKAREN4 + PGK-blast

Plasmid

#167825

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-PE2-IRES-ZeoR

Plasmid

#207352

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

Prime editor 2 (PE2) enzyme

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-N1303K-ngRNA+14_EF1a-puroR

Plasmid

#207359

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

N1303K-CFTR +14 ngRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-L227R-ngRNA+15_EF1a-puroR

Plasmid

#207360

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

L227R-CFTR +15 ngRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pE-N1-RDH11

Plasmid

#161916

Purpose

Expresses untagged human RDH11. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Retinol dehydrogenase 11 (RDH11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV x EF1a_EKAREN4(TA)-ires-blast

Plasmid

#167831

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-HSD17B13-WT-FLAGC

Plasmid

#184501

Purpose

Expresses human HSD17B13 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13 Human)

Use

Tags

FLAGC-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PGL4.11-COL1A1mSMAD

Plasmid

#230917

Purpose

Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)

Use

Luciferase

Tags

Luciferase-luc2p

Expression

Bacterial and Mammalian

Mutation

COL1A1 promoter with mutant potential SMAD bindin…

Promoter

COL1A1

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-HSD17B11-WT-FLAGC

Plasmid

#161903

Purpose

Expresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)

Use

Tags

FLAG-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-CMV-MLH1dn-IRES-puroR

Plasmid

#207354

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

MutL homolog 1 (MLH1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Δ754-756 dominant negative mutant

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-FLAG-BRAT1

Plasmid

#161920

Purpose

Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

BRCA1-associated ATM activator 1 (BRAT1 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-HPGD-FLAGC

Plasmid

#161915

Purpose

Expresses human HPGD with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

HPGD (HPGD Human)

Use

Tags

FLAG-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

Plasmid

#207357

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PGL4.11-COL1A1mTEAD

Plasmid

#230916

Purpose

Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)

Use

Luciferase

Tags

Luciferase- luc2p

Expression

Bacterial and Mammalian

Mutation

mutant potential TEAD bind elements AGGAAT to CTG…

Promoter

COL1A1

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge