We narrowed to 13,942 results for: cas9

1. pSLQ1658-dCas9-EGFP

   Plasmid

   #51023

   Purpose

   Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

   Depositor

   Stanley Qi, Bo Huang

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   dCas9 fuse to EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   MSCV LTR promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpRY dCas9 KRAB

   Plasmid

   #188501

   Purpose

   Expresses FLAG tagged SpRY dCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. nCas9-SpRY_P2A-mCherry

   Plasmid

   #219935

   Purpose

   Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Sp-nCas9(D10A)-SpRY_P2A-mCherry
   

   Use

   CRISPR

   Tags

   SV40 bpNLS and SV40 bpNLS-P2A-mCherry

   Expression

   Mammalian

   Mutation

   nCas9(D10A)

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cp PS Intein dCas9-VPR

   Plasmid

   #242031

   Purpose

   Blue-light-controlled release of dCas9-VPR from the cytoplasm to the nucleus.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

   Insert

   Cp PS Intein dCas9-VPR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fuw-dCas9-Tet1CD

   Plasmid

   #84475

   Purpose

   Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Tet1CD
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-CBE6b-V106W

   Plasmid

   #215825

   Purpose

   Express CBE6b V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-Cas9-GFP

   Plasmid

   #86145

   Purpose

   Lentiviral vector expressing Cas9-GFP

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   Cas9-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ609-dCas9-KR-puro

   Plasmid

   #158478

   Purpose

   Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

   Depositor

   Brendan Price

   Article

   House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

   Insert

   dCas9-KR
   

   Use

   CRISPR

   Tags

   KillerRed

   Expression

   Mammalian

   Mutation

   Cas9m4 (D10A+D839A+H840A+N863A)

   Promoter

   CMV

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-DNMT3A-EGFP

    Plasmid

    #71666

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. eSpCas9(1.1)_No_FLAG_AAVS1_T2

    Plasmid

    #79888

    Purpose

    Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHDR-USR-SpCas9

    Plasmid

    #208829

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SETD2(SET)-Cas9

    Plasmid

    #186700

    Purpose

    Plasmid encoding SETD2-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETD2(SET)-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCENPTΔC-dCas9-3xGFP

    Plasmid

    #198325

    Purpose

    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    CENPTΔC (CENPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP x 3 and dCas9

    Expression

    Mammalian

    Mutation

    truncation - encodes only amino acids 1-375

    Promoter

    CMV-TetO

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-NmCas9-Flag

    Plasmid

    #109030

    Purpose

    Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Tags

    Flag Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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