We narrowed to 1,546 results for: pbluescript

1. pMK446 (BSD-P2A-BromoTag-mClover)

   Plasmid

   #214376

   Purpose

   BromoTag-mClover for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag-mClover
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK447 (BSD-P2A-BromoTag-mCherry)

   Plasmid

   #214377

   Purpose

   BromoTag-mCherry for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

   Plasmid

   #121184

   Purpose

   OsTIR1 introduction

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   CMV-OsTIR1-loxP-PURO-loxP
   

   Use

   CRISPR

   Mutation

   OsTIR1 codon optimized for human cells

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS hTSP-5

   Plasmid

   #13002

   Depositor

   Jack Lawler

   Article

   Newton et al Genomics. 1994 Dec . 24(3):435-9.

   Insert

   COMP (COMP Human)
   

   Expression

   Bacterial

   Available Since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pSAG1::CAS9-U6::sgUPRT

   Plasmid

   #54467

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.

   Depositor

   David Sibley

   Article

   Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

   Insert

   SAG1 5' UTR
   

   Use

   CRISPR; Toxoplasma expression

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-ERN

   Plasmid

   #80475

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Expression

   Mammalian

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY2F

   Plasmid

   #53285

   Purpose

   Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   lcyE (LUT2 Mustard Weed)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAID1.2-EF1a-CGFP-mAID

   Plasmid

   #140608

   Purpose

   Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Expression

   Mammalian

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pfli1

   Plasmid

   #22413

   Depositor

   Nathan Lawson

   Article

   Lawson et al Development. 2001 Oct . 128(19):3675-83.

   Insert

   fli1 (fli1 Zebrafish)
   

   Use

   Riboprobe

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

10. PB-TAG-ERN

    Plasmid

    #80476

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS diaCas9_sgRNA

    Plasmid

    #74923

    Purpose

    Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

    Depositor

    Per Winge

    Article

    Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR

    Promoter

    Cas9 module and sgRNA module

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

    Plasmid

    #158963

    Purpose

    Cre-dependet Cas9-GFP expression

    Depositor

    Michael Brand

    Article

    Kesavan et al Development. 2020 Jun 8;147(11). pii: de

    Inserts

    hsp70l promoter
    floxed Non-FP cassette
    Cas9-GFP
    

    Use

    CRISPR and Cre/Lox

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TAG-ERP2

    Plasmid

    #80479

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. hMSH2 Clone E3 (full-length)

    Plasmid

    #16453

    Depositor

    Bert Vogelstein

    Article

    Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

    Insert

    MSH2 (MSH2 Human)
    

    Expression

    Bacterial

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEED-LoxP-sfGFP:ALFA

    Plasmid

    #219660

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP:ALFA SEED Cassette (LoxP)
    

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK288 (mAID-Bsr)

    Plasmid

    #72826

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Bsr
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-TRE-tdKatushka2-WPRE

    Plasmid

    #193337

    Purpose

    Expresses tdKatushka2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    tdKatushka2
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT346_pLambda-attB-pCA-GFP-pA

    Plasmid

    #52551

    Purpose

    for producing PhiC31 attB-containing minicircle in vitro

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    Lambda integrase attL1
    Lambda integrase attR1
    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBlu/gRNA

    Plasmid

    #59188

    Purpose

    Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Guide RNA Cassette
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6 (Arabidopis)

    Available Since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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