We narrowed to 1,479 results for: pbluescript

1. pMK278 (mClover-Hygro)

   Plasmid

   #72794

   Purpose

   C-terminal tagging with mClover using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mClover-Hygro
   

   Use

   CRISPR

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAID1.2-EF1a-NmCherry-mAID

   Plasmid

   #140603

   Purpose

   Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDctp-mSox10-Mp1

   Plasmid

   #13513

   Depositor

   William Pavan

   Article

   Hakami et al Mech Dev. 2006 Feb . 123(2):124-34.

   Insert

   Sox10 (Sox10 Mouse)
   

   Use

   Tags

   Dct promoter and Mp1-polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pdgfra-T2A-H2B-eGFP donor

   Plasmid

   #113120

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMADM-beta

   Plasmid

   #36891

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   Beta-Geo
   thymidine kinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY2F

   Plasmid

   #53285

   Purpose

   Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   lcyE (LUT2 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWKS7

   Plasmid

   #139414

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. GLI-3 bs-2

   Plasmid

   #16420

   Depositor

   Bert Vogelstein

   Article

   Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

   Insert

   GLI3 (GLI3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pBS Galectin1

   Plasmid

   #19216

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Galectin1 (LGALS1 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pBS NL4-3 envFS eGFP RT-D185K/D186L

    Plasmid

    #101332

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    reverse transcriptase D185K/D186L

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT264

    Plasmid

    #27438

    Depositor

    Liqun Luo

    Article

    Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

    Insert

    TRE-tdTomato-myc flanked by insulators
    

    Use

    Mouse Targeting; Tta dependent

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWKS10

    Plasmid

    #139417

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Clover
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK282 (mCherry2-Bsr)

    Plasmid

    #72798

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Bsr
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS NL4-3 envFS eGFP Δnef/U3

    Plasmid

    #101346

    Purpose

    Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta nef/LTR

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFT1.0

    Plasmid

    #46138

    Depositor

    Wenbiao Chen

    Article

    Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

    Insert

    mCherry
    

    Use

    Cre/Lox; Mutagenic gene trap for zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS AluYa5rescueA70D

    Plasmid

    #78657

    Purpose

    Generates Alu inserts suitable for rescue

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

    Insert

    Ya5rescue cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK446 (BSD-P2A-BromoTag-mClover)

    Plasmid

    #214376

    Purpose

    BromoTag-mClover for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-BromoTag-mClover
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK447 (BSD-P2A-BromoTag-mCherry)

    Plasmid

    #214377

    Purpose

    BromoTag-mCherry for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-BromoTag-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGWKS5

    Plasmid

    #139412

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Citrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSAG1::CAS9-U6::sg290860-6

    Plasmid

    #59855

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

    Depositor

    David Sibley

    Article

    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

    Insert

    CRISPR sg290860-6
    

    Use

    CRISPR; ; toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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