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pCDNA3.1-H-Luc_mito

Plasmid

#113930

Purpose

Plasmid encoding the mitochondrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_mito

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_mito

Plasmid

#113927

Purpose

Plasmid encoding the mitochondrial localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_mito

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_nucl

Plasmid

#113928

Purpose

Plasmid encoding the nuclear localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_nucl

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-NL63-D14

Plasmid

#172666

Purpose

encodes NL63 spike protein, for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

HCNV63gp2 (HCNV63gp2 human coronavirus NL63)

Use

Lentiviral and Retroviral

Tags

NA

Expression

Mammalian

Mutation

c-terminal deletion of 14aa

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA

Plasmid

#235678

Purpose

Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

Depositor

Simon Powell

Article

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3_LuxSit_i_mTagBFP2

Plasmid

#194300

Purpose

Mammalian expression of the de novo luciferase, LuxSit-i

Depositor

David Baker, Andy Yeh

Article

Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

Insert

LuxSit-i

Tags

mTagBFP2

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcdna3.1_mouseST6galI

Plasmid

#178435

Purpose

murine St6gal1 expression plasmid

Depositor

Deborah Fass

Article

Ilani et al EMBO J. 2022 Oct 17:e111869. doi: 10.152

Insert

St6gal1 (St6gal1 Mouse)

Tags

6xHIS-TEV Cleavage Site

Expression

Mammalian

Mutation

Coding sequnce for amino acids 85-403

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CCR5

Plasmid

#98943

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3_NSlmb-vhhGFP4

Plasmid

#35579

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Mammalian

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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c-Flag pcDNA3

Plasmid

#20011

Purpose

This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Pu.1

Plasmid

#66974

Purpose

Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Spi1 (Spi1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)_mtZFN(-)2G

Plasmid

#62799

Purpose

mtZFN2G architecture bearing (-) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(-)2G

Tags

Internal FLAG tag immediately after F1B mitochond…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(+)2G

Plasmid

#62798

Purpose

mtZFN2G architecture bearing (+) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(+)2G

Tags

Internal HA tag immediately after F1B mitochondri…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

Plasmid

#176060

Purpose

Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)

Tags

3xFLAG

Expression

Mammalian

Mutation

K164A and H165A

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

Plasmid

#176059

Purpose

Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)

Tags

3xFLAG

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

Plasmid

#218461

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )

Expression

Mammalian

Mutation

Thr1273AGlu substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

Plasmid

#218462

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )

Expression

Mammalian

Mutation

Thr1273Asp substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-spikeRBD::∆ACE2 SURF

Plasmid

#206957

Purpose

Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

Depositor

Xiaokun Shu

Article

Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

Inserts

sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
ACE2 (ACE2 Human)

Tags

cSURF, mCherry, and nSURF

Expression

Mammalian

Mutation

17-615 and RBD (319-541)

Promoter

CMV

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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