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Plasmid#231923PurposeFor expression of MERS-CoV spike protein with glycan deletion at N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutationDepositorInsertspike last4KO (S MERS-CoV)
UseTagsExpressionMammalianMutationN1241Q, N1256Q, N1277Q, N1288QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G1 mutant
Plasmid#231913PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098 by implementing Asn-to-Gln mutationDepositorInsertspike G1 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G12 mutant
Plasmid#231914PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutationDepositorInsertspike G12 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1134QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
BII-C3H
Plasmid#133386PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-B3HTK
Plasmid#133389PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-WT-VWF
Plasmid#124794PurposeExpresses multimeric human VWF with C-terminal c-Myc tagDepositorInsertVWF (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1
Plasmid#133041PurposeSiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-UGCG-KO
Plasmid#80010PurposegRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.DepositorInsertUGCG (UGCG Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
S1-Fc
Plasmid#164220PurposeExpresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporterDepositorInsertSpike S1 domain (S SARS-CoV)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIREN-RetroQ-shLuc
Plasmid#73666PurposepSRIEN-RetroQ vector containing control sequence luciferaseDepositorInsertshRNA to Luciferase
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
SiC-V1-Scr
Plasmid#133042PurposeSiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Cas9-P2A-dTomato-PS9
Plasmid#231911PurposeFor packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signalDepositorInsertCas9-P2A-dTomato
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Cas9-P2A-dTomato-T20
Plasmid#231912PurposeFor packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signalDepositorInsertCas9-P2A-dTomato
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9
Plasmid#231907PurposeFor packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-P2A-dTomato-T2A-TagBFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Const.4 EGFP-IRES-Luc-PS9
Plasmid#231908PurposeFor packaging EGFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-IRES-Luc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2
Plasmid#231904PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging firefly luciferase mRNA into the VLPsDepositorUseLentiviralTagsExpressionMutationR203M in N proteinPromoterCMV, EF-1aAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G12345 mutant
Plasmid#231920PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134, N1158, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G12345 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1134Q, N1158Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2.EGFP
Plasmid#231905PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPsDepositorUseLentiviralTagsExpressionMutationR203M in N proteinPromoterCMV, EF-1aAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G1345 mutant
Plasmid#231919PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G1345 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1158Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
MERS-CoV spike all7KO mutant
Plasmid#231924PurposeFor expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225, N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutationDepositorInsertspike all7KO (S MERS-CoV)
UseTagsExpressionMammalianMutationN1176Q, N1213Q, N1225Q, N1241Q, N1256Q, N1277Q, N…PromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only