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pKCAG-H2BmCherry-OptoJNKi

Plasmid

#89741

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Optogenetics

Tags

Histone2B and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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DAPK1_HUMAN_D0

Plasmid

#79728

Purpose

This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK1 (DAPK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
BMPR2_HUMAN_D0

Plasmid

#79709

Purpose

This plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

BMPR2 (BMPR2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pmCherry-3xNLS-OptoJNKi-dsm

Plasmid

#89743

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi dark-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi-lsm

Plasmid

#89742

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi lit-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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KC1E_HUMAN_D0

Plasmid

#79724

Purpose

This plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1E (CSNK1E Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DAPK3_HUMAN_D0

Plasmid

#79721

Purpose

This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK3 (DAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHB3_HUMAN_D0

Plasmid

#79732

Purpose

This plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB3 (EPHB3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHB2_HUMAN_D0

Plasmid

#79697

Purpose

This plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB2 (EPHB2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Dscaml1-AP-His

Plasmid

#71946

Purpose

Expresses the extracellular region of the Dscaml1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscaml1 (Dscaml1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-MKP7_K55R_153

Plasmid

#37794

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Tags

His

Expression

Bacterial

Mutation

changed Lysnie 55 to Arginine and deleted amino a…

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
MK11_HUMAN_D0

Plasmid

#79692

Purpose

This plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK11 (MAPK11 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
FES_HUMAN_D0

Plasmid

#79686

Purpose

This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FES (FES Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAPK3_HUMAN_D0

Plasmid

#79702

Purpose

This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MAPK3 (MAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
tdEos-Keratin-17

Plasmid

#57629

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Keratin (KRT18 Human)

Tags

tdEos

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Jam2-AP-His

Plasmid

#71953

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Islr2.b-AP-His

Plasmid

#71951

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr2.b (Islr2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-luciferase

Plasmid

#223188

Purpose

Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

luciferase (LOC116160065 firefly)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts1

Plasmid

#174265

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts2

Plasmid

#174266

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge