We narrowed to 53,429 results for: plasmid

1. SEC14L3

   Plasmid

   #73240

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SEC14L3 (SEC14L3 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

2. SEC14L4

   Plasmid

   #73233

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SEC14L4 (SEC14L4 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. p(+)MV-NSE-FlagP-F497A-3p

   Plasmid

   #58796

   Purpose

   Expresses recombinant measles virus with Flag-P-F497A gene tagged si2 (siP) target sequence

   Depositor

   Branka Horvat

   Insert

   Measles virus antigenome
   

   Use

   Tags

   Flag

   Expression

   Mutation

   MeV-P F497A

   Promoter

   T7

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330 Mouse 3' Hp1a gRNA

   Plasmid

   #127898

   Purpose

   WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene

   Depositor

   Mark Groudine

   Insert

   gRNA/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

   Plasmid

   #160629

   Purpose

   TU for the expression of LbCas12a

   Depositor

   Diego Orzaez

   Insert

   AtUBQ10-:LbCpf1(Cas12a):Tnos
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   UBQ10

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-HA-Bax

   Plasmid

   #191944

   Purpose

   Expression of HA-Bax.

   Depositor

   Luca Scorrano

   Insert

   HA-Bax (Bax Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. FU_tetO_dCas9-VP192-T2A-EGFP

   Plasmid

   #121173

   Purpose

   Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

   Depositor

   Timo Otonkoski

   Insert

   dCas9-VP192-T2A-EGFP
   

   Use

   CRISPR and Lentiviral; Inducible by doxycycline

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIX403-EBFP2-ccdB-NatMx

   Plasmid

   #158559

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. DFNB31-TwinStrepII

   Plasmid

   #83196

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   DFNB31 (Whrn Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

    Plasmid

    #51656

    Purpose

    hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

    Depositor

    Adam Cohen

    Insert

    Quasar2-mOrange-Chr64-GFP
    

    Use

    Lentiviral

    Tags

    GFP and mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. acat 1-906

    Plasmid

    #24194

    Depositor

    David Rimm

    Insert

    alpha catenin (CTNNA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full length

    Promoter

    Available Since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR8/GW/TOPO PCGF2

    Plasmid

    #69791

    Purpose

    Gateway entry vector for PCGF2

    Depositor

    Gerard Cagney

    Insert

    PCGF2 (PCGF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET28a_PTK2b

    Plasmid

    #224237

    Purpose

    Expression of human PTK2b Kinase in E. coli

    Depositor

    Andrew Mesecar

    Insert

    ptk2b (PTK2B Human)
    

    Use

    Tags

    6xHis and 6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD400

    Plasmid

    #137024

    Purpose

    SapTrap mNG-GLO donor

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    C. elegans GLO-optimized mNeonGreen
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. YEpADE1

    Plasmid

    #22495

    Depositor

    Beth Montelone

    Insert

    ADE1 (ADE1 Budding Yeast)
    

    Use

    Yeast shuttle

    Tags

    Expression

    Mutation

    Original 4067 bp HindIII-HindIII insertion (see Y…

    Promoter

    Available Since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBHM2642

    Plasmid

    #229526

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET11a_PTK2b_Kinase

    Plasmid

    #224242

    Purpose

    Expression of human PTK2b Kinase domain in E. coli

    Depositor

    Andrew Mesecar

    Insert

    ptk2b_kinase (PTK2B Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLIX403-EYFP-ccdB-Blast

    Plasmid

    #158551

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-WT-long-UBASH3A-Ntag

    Plasmid

    #192098

    Purpose

    Human wild-type long-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221-WT-long-UBASH3A-Ctag

    Plasmid

    #192097

    Purpose

    Human wild-type long-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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