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pRL2960

Plasmid

#70694

Purpose

pDU1-E. coli shuttle vector (Km-r) with extended polylinker

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pTn7xKS-mPlum

Plasmid

#117396

Purpose

Tn7 tagging vector pTn7xKS with mPlum expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

mPlum

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJZC523

Plasmid

#62320

Purpose

sgRNA for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Expression

Yeast

Promoter

SNR52

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E1E2 (20–629)

Plasmid

#132627

Purpose

expresses human LRP6 E1E2 (20–629) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (20–629)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-Firefly

Plasmid

#79495

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Firefly Luciferase

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 9

Plasmid

#8476

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
pRC01

Plasmid

#114680

Purpose

sfGFP.Strands 1-9

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP Strands 1-9

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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rNL1(46-638)

Plasmid

#20172

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neuroligin 1 (Nlgn1 Rat)

Tags

C-terminal 6X His tag

Expression

Insect

Mutation

no splice sites

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only