We narrowed to 65,548 results for: nin
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Plasmid#169187PurposeTo express His-SUMO-nsp8 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
Tags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP3-bio
Plasmid#47731PurposeExpresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP3
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-SIG-5HTR4D100A
Plasmid#16312DepositorInserth5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)
TagsFLAGExpressionMammalianMutationAdded FLAG tag; Aspartic Acid 100 changed to Alan…Available SinceJan. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-miniDq-P2A-mCherry
Plasmid#204357PurposeAAV vector for miniDq expression under the control of human synapsin promoterDepositorInsertminiDq-P2A-mCherry
UseAAVTagsmCherryMutationThe third intracellular loop (ICL3) of hM3Dq was …PromoterhSynAvailable SinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-ddMSK1
Plasmid#165603PurposeExpresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)DepositorInsertS. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 S. Pyogenes, Human, Synthetic)
UseCRISPR and LentiviralTagsFLAG TagExpressionMammalianPromoterEF1aAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nef-CIAO-Flp
Plasmid#149294PurposeCre-dependent expression of Flp recombinase.DepositorInsertFlp recombinase (flp Budding Yeast)
UseAAVMutationDerived from the mouse codon-optomized FlpO. The…Available SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-GABARAP-GFP
Plasmid#123111PurposeExpresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
Tags3xFLAG and EGFPExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1
Plasmid#122084PurposeExpress M.EcoGII fused to TRF1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-TERF1
UseRetroviralTagsV5 tag on Nter of TERF1ExpressionBacterial and MammalianAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
ExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…Available SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMVS102_P3-GFP-NATMX6
Plasmid#99048PurposeEGFP reporter with six binding sites for the Zif268 DNA binding domainDepositorInsertsMcIsaac 2014 P3 promoter
eGFP
clonNAT resistance
ExpressionYeastMutationGAL1 promoter with 4 GAL4 sites removed and repla…PromoterMcIsaac 2014 P3 promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2high-mCherry-Raf1
Plasmid#104064PurposeExpresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and Raf1DepositorExpressionMammalianMutationCRY2PHR E490RPromoterCMVAvailable SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-PRDX5WT-VA
Plasmid#98688PurposeLentiviral expression of human PRDX5-WT in mammalian cellsDepositorAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
SHP2(C459S)@pLKO-Trex-HA
Plasmid#85459PurposeExpresses SHP2 mutant in mammalian cellsDepositorAvailable SinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-F1888L
Plasmid#69010Purposeactivating MTOR mutationDepositorInsertMTOR-F1888L (MTOR Human)
TagsFLAGExpressionMammalianMutationchange Phenylalanine 1888 to LeucineAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNATM3.1/V5-His TOPO-PKCi-K274W
Plasmid#55690Purposeexpresses dominant negative human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
TagsV5/HisExpressionMammalianMutationA120E and K274WPromoterCMVAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-VMA1 chr I
Plasmid#41896PurposeYeast expression of VMA1.DepositorInsertVMA1 (VMA1 Budding Yeast)
ExpressionYeastMutationContains silent mutation at 342A-G and L87S mutat…PromoterGPDAvailable SinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)
Plasmid#44094DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…Available SinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-mEF
Plasmid#31765DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneMutationMutated in both EF-hand domains: D231A, E242K, D4…Available SinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag SPHK2
Plasmid#20571DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin
Plasmid#133025PurposeExpresses EGFP-tagged tail anchor sequence of monoamine oxidase ADepositorInsertMonoamine oxidase type A (MAOA Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-488PromoterHuman elongation factor 1 alphaAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only