We narrowed to 10,590 results for: Kars

1. pU6-sgCD71-2

   Plasmid

   #46918

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgCD71 -2 (TFRC Human)
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas-Cas12m

   Plasmid

   #192274

   Purpose

   Encodes MmCas12m under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23108

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF1a-hMLH1dn (og codon)

   Plasmid

   #174823

   Purpose

   Mammalian expression of human MLH1dn (original codon usage)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (original codon usage) (MLH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-v2 RsTAL At4CL

   Plasmid

   #126524

   Purpose

   expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

   Depositor

   Andrew Woolley

   Article

   Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

   Inserts

   tyrosine ammonia lyase
   p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. BTG1

   Plasmid

   #155425

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   BTG1 (BTG1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-MS2V5-eRF3a F76a

   Plasmid

   #65811

   Purpose

   expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

   Depositor

   Niels Gehring

   Article

   Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

   Insert

   eRF3a (GSPT1 Human)
   

   Use

   Tags

   MS2 stem loops and V5

   Expression

   Mammalian

   Mutation

   F76a

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a_SOX2_P2A_Hygro_Barcode

   Plasmid

   #120480

   Purpose

   Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBZCas13b

   Plasmid

   #89898

   Purpose

   Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX302_HOXB13

   Plasmid

   #70089

   Purpose

   Expression of human HOXB13 from CMV promoter, puromycin selection marker

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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