We narrowed to 10,053 results for: ADA

1. pTT3 Unc5A-FLAG

   Plasmid

   #72194

   Purpose

   Expresses full-length Unc5a with a C-terminal FLAG tag

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5a (Unc5a Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-itis pBE-t

   Plasmid

   #195342

   Purpose

   Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

   Depositor

   Alexis Komor

   Article

   Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

   Inserts

   ecTadA(8e)-SpCas9-NG
   gRNA ctctacgcgggtcttgtagt
   

   Use

   CRISPR

   Mutation

   SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

   Promoter

   Lac and ProC

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC18T-mini-Tn7T-Gm-Pc-mCherry

   Plasmid

   #199247

   Purpose

   Fluorescent labelling of bacterial cells with mCherry

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized mCherry gene
   

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-itis pBE-nt

   Plasmid

   #195343

   Purpose

   Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

   Depositor

   Alexis Komor

   Article

   Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

   Inserts

   ecTadA(8e)-SpCas9-NG
   gRNA gtgcacgacgccgtatgcga
   

   Use

   CRISPR

   Mutation

   SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

   Promoter

   Lac and ProC

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HT103_FCMV_QuasAr6a_Citrine

   Plasmid

   #178822

   Purpose

   Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6a carrying a Citrine expression tag

   Depositor

   Adam Cohen

   Article

   Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

   Insert

   QuasAr6a_Citrine
   

   Use

   Lentiviral

   Tags

   citrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a

   Plasmid

   #31483

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
   

   Use

   Lentiviral

   Mutation

   Traffic Light 2.0 series have a +3 PEST sequence …

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTT3 Unc5D-FLAG

   Plasmid

   #72197

   Purpose

   Expresses full-length Unc5d with a C-terminal FLAG tag

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5d (Unc5d Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. HT109_pAAV_hSyn-DiO-SomQuasAr6a_EGFP

   Plasmid

   #190878

   Purpose

   Cre-on expression of soma-targeted QuasAr6a under an neuronal promoter

   Depositor

   Adam Cohen

   Article

   Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

   Insert

   somQuasAr6a_EGFP
   

   Use

   AAV

   Tags

   EGFP

   Promoter

   hSyn

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEosEM-E_Lamin A

   Plasmid

   #226338

   Purpose

   Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression

   Depositor

   Pingyong Xu

   Article

   Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

   Insert

   Lamin A (LMNA Human)
   

   Tags

   mEosEM-E

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBSFI-myr-akt-T308A/S473A

    Plasmid

    #49191

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Mutation

    T308A; S473A

    Promoter

    T7

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. DRH335: FCK-Optopatch1

    Plasmid

    #51695

    Purpose

    Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    Optopatch1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-beta1(617-949)-GFP

    Plasmid

    #100732

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP1B1 (AP1B1 Human)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. HT028_FCMV_CheRiff_EGFP

    Plasmid

    #178821

    Purpose

    Expresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    CheRIff_EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pαH-S-RRAR

    Plasmid

    #164569

    Purpose

    SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

    Plasmid

    #61841

    Purpose

    mammalian expression of cyclin B1 non-degradable L45A mutant

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    cyclin B1 (CCNB1 Human)
    

    Tags

    2xSTREP and 3xFLAG

    Expression

    Mammalian

    Mutation

    L45A

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. ABE8.17-d

    Plasmid

    #136299

    Purpose

    expresses ABE8.17-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pblic-neo TRX1

    Plasmid

    #110919

    Purpose

    To overexpress Thioredoxin-1

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Retroviral

    Promoter

    unknown

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. HTRA1_PDZ_1

    Plasmid

    #103912

    Purpose

    Protein expression and purification of HTRA1 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    HTRA1 (HTRA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18T-mini-Tn7T-Gm-Pc-mKate2

    Plasmid

    #199249

    Purpose

    Fluorescent labelling of bacterial cells with mKate2

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized mKate2 gene
    

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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