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p2T-CMV-evoCjCas9-eAID-BlastR

Plasmid

#194062

Purpose

Mammalian expression of evoCjCas9 eAID

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF-L1-dMb2Cas12a

Plasmid

#177683

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dMb2Cas12a

Use

CRISPR; Gateway compatible foki-dmb2cas12a entry …

Tags

NLS

Expression

Plant

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i-Max-T2A-EGFP

Plasmid

#188268

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-Max

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i-HiFi-T2A-EGFP

Plasmid

#188269

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-HiFi

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ131C2.0

Plasmid

#99886

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ132C2.0

Plasmid

#99889

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-NBid-PhoCl1-CBid

Plasmid

#164050

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl1

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl1-CBid

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pNb4OMT-A53M

Plasmid

#216232

Purpose

Expresses a mutant Norbelladine 4'-O-Methyltransferase

Depositor

Simon d'Oelsnitz, Andrew Ellington

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Insert

Nb4OMT-A53M

Use

Synthetic Biology

Mutation

A53M

Promoter

P150

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEVOL-MmAcKRS1-PylTUUA

Plasmid

#182537

Purpose

N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppression

Depositor

Wenshe Liu

Article

Wu et al Chembiochem. 2012 Jul 9;13(10):1405-8. d

Insert

PylTUUA

Expression

Bacterial

Promoter

ProK

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKMV-VioE

Plasmid

#65351

Purpose

systhesis of vioE gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioE

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pX601‐mhCMV‐miniABEmax‐N2‐ E53ogRNA

Plasmid

#187065

Purpose

Gp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmax-Gp41-1

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mhCMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA

Plasmid

#187066

Purpose

Gp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

Gp41-1-ABEmaxNGA

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mhCMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry_eDHFR_SIM#1

Plasmid

#164646

Purpose

tag SIM with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIM

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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GAE teo-GFP

Plasmid

#83958

Purpose

Can be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

TetO-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF05

Plasmid

#52558

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF05

Expression

Insect

Mutation

changed from GCaMP3: D397N/G398A/N399D

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pKMV-VioA

Plasmid

#65347

Purpose

systhesis of vioA gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioA

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pBW414hrpL-cIgfp

Plasmid

#61439

Purpose

pSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs34-CI-LVA-t-plam-30gfp-t

Tags

LVA

Expression

Bacterial

Promoter

HrpL

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-eDHFR-SIMvada

Plasmid

#164649

Purpose

SIM mutant tagged with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIMvada

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-miR122 sponge

Plasmid

#35665

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

U6 miR-122sponge

Promoter

U6

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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