We narrowed to 18,078 results for: comp

1. pAAVsc.U6-sgAspa.CMV/CB-EGFP

   Plasmid

   #121510

   Purpose

   Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgAspa
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACEBAC1-CoREST-C

   Plasmid

   #109159

   Purpose

   Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available Since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

   Plasmid

   #115432

   Purpose

   Vector for expressing RPL28 with mutagenized exon2

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   exon 2 deleted, with MfeI / NheI cloning site

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFL I3

   Plasmid

   #113514

   Purpose

   Expression of I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac-LacI-LAP-HEC1

   Plasmid

   #114033

   Purpose

   Production of baculovirus with LacI-LAP-HEC1

   Depositor

   Geert Kops

   Article

   Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

   Insert

   LacI-LAP-HEC1 (NDC80 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   none

   Promoter

   CMV

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIG1_UL136_19kDa_EE

   Plasmid

   #74951

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Glu-Glu tag (EYMPME)

   Expression

   Mammalian

   Mutation

   delta 1-127

   Promoter

   CMV, lentiviral LTR

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIG1_UL136_23kDa_Myc

   Plasmid

   #74949

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   HA Tag (YPYDVPDYA)

   Expression

   Mammalian

   Mutation

   delta 1-99 M128A

   Promoter

   CMV, Lentiviral LTR

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-biotin-myc- mCE

   Plasmid

   #82475

   Purpose

   Expresses mRNA capping enzyme containing N terminal biotin and myc tag

   Depositor

   Daniel Schoenberg

   Article

   Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

   Insert

   Biotin (Rngtt Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_6HT-TBP6.7(Q48T)

    Plasmid

    #112725

    Purpose

    This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48T)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48T

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_6HT-TBP6.7(P51A)

    Plasmid

    #112728

    Purpose

    Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P51A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P51A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHH0103_BZRAP1-2/3

    Plasmid

    #91551

    Purpose

    Protein expression and purification of human SH3 domain construct BZRAP1-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BZRAP1-2/3 (TSPOAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_SH3RF2-2/3

    Plasmid

    #91557

    Purpose

    Protein expression and purification of human SH3 domain construct SH3RF2-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3RF2-2/3 (SH3RF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_NOXO1-2/2

    Plasmid

    #91558

    Purpose

    Protein expression and purification of human SH3 domain construct NOXO1-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NOXO1-2/2 (NOXO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_BZRAP1-3/3

    Plasmid

    #91559

    Purpose

    Protein expression and purification of human SH3 domain construct BZRAP1-3/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BZRAP1-3/3 (TSPOAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_GRAP-2/2

    Plasmid

    #91529

    Purpose

    Protein expression and purification of human SH3 domain construct GRAP-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRAP-2/2 (GRAP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_PIK3R2-1/1

    Plasmid

    #91535

    Purpose

    Protein expression and purification of human SH3 domain construct PIK3R2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PIK3R2-1/1 (PIK3R2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pHH0103_NCK2-1/3-SV1/2

    Plasmid

    #91538

    Purpose

    Protein expression and purification of human SH3 domain construct NCK2-1/3-SV1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK2-1/3-SV1/2 (NCK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge